AI Article Synopsis
- B-type natriuretic peptide (BNP) is a key biomarker for diagnosing heart failure, and this study introduces a new electrochemical biosensor for its detection.
- The biosensor utilizes a combination of CRISPR/Cas13a technology, a DNA aptamer for specific BNP binding, and signal amplification through T7 RNA polymerase, achieving high sensitivity (detection limit of 0.74 aM) and specificity.
- Tested on human serum samples, the biosensor showed minimal interference, highlighting its potential for practical clinical use and as a point-of-care diagnostic tool.
Article Abstract
B-type natriuretic peptide (BNP) is a biomarker for heart failure, a serious and prevalent disease that requires rapid and accurate diagnosis. In this study, we developed a novel electrochemical biosensor for BNP detection based on CRISPR/Cas13a and chain substitution reaction. The biosensor consists of a DNA aptamer that specifically binds to BNP, a T7 RNA polymerase that amplifies the signal, a CRISPR/Cas13a system that cleaves the target RNA, and a two-dimensional DNA nanoprobe that generates an electrochemical signal. The biosensor exhibits high sensitivity, specificity, and stability, with a detection limit of 0.74 aM. The biosensor can also detect BNP in human serum samples with negligible interference, demonstrating its potential for clinical and point-of-care applications. This study presents a novel strategy for integrating CRISPR/Cas13a and chain substitution reaction into biosensor design, offering a versatile and effective platform for biomolecule detection.
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| http://dx.doi.org/10.1016/j.talanta.2024.125966 | DOI Listing |
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