AI Article Synopsis
- 5-Aminolevulinic acid (5-ALA) is essential for making heme and chlorophyll, and this study focuses on creating efficient microbial production systems for it.
- Researchers identified a novel 5-ALA synthase and used a small RNA library to enhance production through specific gene repression, resulting in significant increases in 5-ALA levels.
- By employing strategies to improve efflux and reduce oxidative stress, they developed a microbial strain capable of producing up to 6.19 g·L of 5-ALA, advancing methods for its bioproduction.
Article Abstract
5-Aminolevulinic acid (5-ALA) plays a pivotal role in the biosynthesis of heme and chlorophyll and has garnered great attention for its agricultural applications. This study explores the multifaceted construction of 5-ALA microbial cell factories. Evolutionary analysis-guided screening identified a novel 5-ALA synthase from as the best synthase. An sRNA library facilitated global gene screening that demonstrated that and repression enhanced 5-ALA production by 74.3% and 102%, respectively. Subsequently, efflux of 5-ALA by the transporter Gdx increased 5-ALA biosynthesis by 25.7%. To mitigate oxidative toxicity, DNA-binding proteins from starved cells were employed, enhancing cell density and 5-ALA titer by 21.1 and 4.1%, respectively. Combining these strategies resulted in an strain that produced 5-ALA to 1.51 g·L in shake flask experiments and 6.19 g·L through fed-batch fermentation. This study broadens the repertoire of available 5-ALA synthases and transporters and provides a new platform for optimizing 5-ALA bioproduction.
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| http://dx.doi.org/10.1021/acs.jafc.4c00903 | DOI Listing |
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