AI Article Synopsis
- - The study investigates pulmonary arterial hypertension (PAH), focusing on the early activation of pulmonary arterial smooth muscle cells (PASMCs) and the mechanisms behind it.
- - Using data from the Gene Expression Omnibus (GEO) and advanced analysis techniques, researchers identified 46 key genes related to PAH, with MACC1 emerging as a crucial gene linked to the disease's progression.
- - The research indicates that targeting MACC1 could be a potential treatment strategy for PAH, suggesting the need for further clinical studies to evaluate its effectiveness.
Article Abstract
Background: Pulmonary arterial hypertension (PAH) is a life-threatening disease characterized by abnormal early activation of pulmonary arterial smooth muscle cells (PASMCs), yet the underlying mechanisms remain to be elucidated.
Methods: Normal and PAH gene expression profiles were obtained from the Gene Expression Omnibus (GEO) database and analyzed using gene set enrichment analysis (GSEA) to uncover the underlying mechanisms. Weighted gene co-expression network analysis (WGCNA) and machine learning methods were deployed to further filter hub genes. A number of immune infiltration analysis methods were applied to explore the immune landscape of PAH. Enzyme-linked immunosorbent assay (ELISA) was employed to compare MACC1 levels between PAH and normal subjects. The important role of MACC1 in the progression of PAH was verified through Western blot and real-time qPCR, among others.
Results: 39 up-regulated and 7 down-regulated genes were identified by 'limma' and 'RRA' packages. WGCNA and machine learning further narrowed down the list to 4 hub genes, with MACC1 showing strong diagnostic capacity. In vivo and in vitro experiments revealed that MACC1 was highsly associated with malignant features of PASMCs in PAH.
Conclusions: These findings suggest that targeting MACC1 may offer a promising therapeutic strategy for treating PAH, and further clinical studies are warranted to evaluate its efficacy.
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| http://dx.doi.org/10.1016/j.compbiomed.2024.108372 | DOI Listing |
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