The and anti-inflammatory and anti-oxidative effects of an amino acid blend supplemented feed on pigs experimentally challenged with .

Background: The and anti-inflammatory and anti-oxidative effects of an amino acid (AA) blend (tryptophan, threonine, and methionine) in pigs.

Objective: This study aimed to evaluate the anti-inflammatory and anti-oxidative effects of an AA blend on intestinal porcine epithelial cells (IPEC-J2) and the anti-inflammatory and anti-oxidative effects in pigs experimentally challenged with .

Methods: IPEC-J2 were pretreated with an AA blend for 25 h and then treated with lipopolysaccharide (LPS), deoxynivalenol (DON), or HO for evaluation. A controlled standard diet supplemented with 0.3% of the AA blend was orally fed to the treated group pigs for 14 days, beginning at 21 days of age. At the end of the feeding period, pigs were orally inoculated with .

Results: Pre-treatment with the AA blend reduced LPS/DON-induced interleukin (IL)-8 mRNA as a measurement of the anti-inflammatory effect and HO-induced reactive oxygen species (ROS) as a measurement of the anti-oxidative effect on IPEC-J2. Feeding with an AA blend resulted in a reduction of proinflammatory (tumor necrosis factor-α, IL-6, and IL-8) cytokine levels, while treated pigs experienced an increase in anti-inflammatory IL-10 cytokine in their sera. The addition of an AA blend-supplemented pig feed resulted in significantly lower -induced cecal lesion scores compared to untreated pigs.

Discussion: Supplementation of feed with an AA blend reduced intestinal inflammation and pathology in pigs and may be applied for the control of infection, as demonstrated in this study.