AI Article Synopsis
- The study focuses on the role of a specific gene related to PPR proteins in rice, which is crucial for chloroplast development and photosynthesis.
- Researchers identified a mutant rice plant with pale green leaves, indicating reduced chlorophyll and abnormal chloroplast development compared to the wild type.
- Using CRISPR/Cas9 gene editing, they confirmed that the identified gene affects RNA editing and splicing within chloroplasts, highlighting its importance in regulating chloroplast function in rice plants.
Article Abstract
Unlabelled: The chloroplast serves as the primary site of photosynthesis, and its development plays a crucial role in regulating plant growth and morphogenesis. The Pentatricopeptide Repeat Sequence (PPR) proteins constitute a vast protein family that function in the post-transcriptional modification of RNA within plant organelles. In this study, we characterized mutant of rice with pale green leaves (). The chlorophyll content of at the seedling stage was significantly reduced compared to the wild type (WT). Transmission electron microscopy (TEM) and quantitative PCR analysis revealed that exhibited aberrant chloroplast development compared to the wild type (WT), accompanied by significant alterations in gene expression levels associated with chloroplast development and photosynthesis. The Mutmap analysis revealed that a single base deletionin the coding region of in . By employing CRISPR/Cas9 mediated gene editing, two homozygous - mutants were generated and exhibited a similar phenotype to , thereby confirming that was responsible for Consequently, it was designated as . belongs to the DYW-type PPR protein family and is localized in chloroplasts. Furthermore, we demonstrated that the RNA editing efficiency of and , and the splicing efficiency of were significantly decreased in mutants compared to WT. Collectively, these results indicate that plays a crucial role in chloroplast development by regulating the editing and splicing of chloroplast genes in rice.
Supplementary Information: The online version contains supplementary material available at 10.1007/s11032-024-01468-7.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10965880 | PMC |
| http://dx.doi.org/10.1007/s11032-024-01468-7 | DOI Listing |
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