AI Article Synopsis
- - The study investigates the role of TCTP (translationally controlled tumor protein) in regulating oncogenic intercellular signaling through extracellular vesicles (EVs), particularly in response to genotoxic stress.
- - Researchers used a Tctp-inducible knockout mouse model and found that TCTP is crucial for triggering apoptosis signaling and promoting malignant growth via small EVs (sEVs).
- - The findings suggest that TCTP enhances tumor development by binding to DDX3 and recruiting RNAs, including miRNAs, to sEVs, and inhibiting TCTP can improve survival rates in tumor-prone mice.
Article Abstract
Oncogenic intercellular signaling is regulated by extracellular vesicles (EVs), but the underlying mechanisms remain mostly unclear. Since TCTP (translationally controlled tumor protein) is an EV component, we investigated whether it has a role in genotoxic stress signaling and malignant transformation. By generating a Tctp-inducible knockout mouse model (Tctp), we report that Tctp is required for genotoxic stress-induced apoptosis signaling via small EVs (sEVs). Human breast cancer cells knocked-down for TCTP show impaired spontaneous EV secretion, thereby reducing sEV-dependent malignant growth. Since Trp53 mice are prone to tumor formation, we derived tumor cells from Trp53;Tctp double mutant mice and describe a drastic decrease in tumori-genicity with concomitant decrease in sEV secretion and content. Remarkably, Trp53;Tctp mice show highly prolonged survival. Treatment of Trp53 mice with sertraline, which inhibits TCTP function, increases their survival. Mechanistically, TCTP binds DDX3, recruiting RNAs, including miRNAs, to sEVs. Our findings establish TCTP as an essential protagonist in the regulation of sEV-signaling in the context of apoptosis and tumorigenicity.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11014985 | PMC |
| http://dx.doi.org/10.1038/s44319-024-00108-7 | DOI Listing |
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