Fabrication of a 96-electrode array using carbon dioxide laser ablation.

The 96 laser-induced multigraphene electrode (96L-MGE) integrated microwell plate (96 L-MGE-MP) is described. Each cell includes separate working, auxiliary, and reference electrodes, and the array sits on a poly-methyl methacrylate (PMMA) well. The 96 electrochemical cells were fabricated by laser ablation of polyimide adhesive tape, which created laser-induced graphene electrodes (L-GE). The microwell was produced using laser ablation of the PMMA sheet as well. The morphology and electrochemical characterization of L-GE were controlled by tuning the laser processing. L-GE fabricated at laser power-laser speed ratios of 0.008-0.02 W s mmdisplayed good electrochemical behaviors. Under the optimal condition of L-GE fabrication, the measured L-GE surface roughness was 475.47 nm. The 96 L-MGE can be fabricated in 24.2 min and is compatible with various analytes. 10 benchmark redox compounds were shown as electrocatalytic examples. The performance of each analyte was investigated by voltammetry. As proof of concept, 96 L-MGE-MP was connected to a 96× connector for multichannel detection. The RSD of the 96 L-MGE-MPwas below 5.3%, which demonstrated good fabrication reproducibility.