AI Article Synopsis
- Traditional antioxidant screening often uses chemical tests to evaluate a compound's ability to fight oxidation, but these results don’t easily translate to how they work in complex biological systems.
- This study examined fruit-derived phenolic compounds (hyperoside, epicatechin, and phlorizin) through various experiments, revealing that these compounds had varying antioxidant activities, with hyperoside showing the most effectiveness.
- Importantly, the combinations of these compounds demonstrated a synergistic effect, particularly in protecting plasma from oxidation, suggesting their potential health benefits while indicating that they didn’t damage human cells even at high concentrations.
Article Abstract
Traditional antioxidant screening relies on chemical assays to assess compounds' potential in combating oxidative processes. However, translating chemical antioxidant activity to complex biological systems poses challenges. In this study, the antioxidant potential of fruit-derived phenolic compounds, hyperoside (HP), epicatechin (EC), and phlorizin (PZ), and their combinations in a specific ratio were investigated using a simplex-centroid design of experiments. The research included antioxidant assays, plasma protection against oxidation tests, and cytotoxicity assessments in human cell lines. The results revealed the complex relationship between chemical antioxidant activity and its relevance to cellular oxidative and antioxidative processes. HP and EC exhibited significant antioxidant activity, with HP outperforming EC in multiple assessments. Cytotoxicity assay confirmed that these compounds did not induce cell death or hinder proliferation, even at higher concentrations (>100 μmol/mL). In the cell antioxidant activity (CAA) test, HP and EC exhibited higher CAA, while PZ displayed lower antioxidant activity. In conclusion, a synergistic effect emerged when HP, EC, and PZ were combined, particularly in plasma protection, suggesting protective effects and potential health benefits. This research emphasized the need for a nuanced understanding of the interplay between chemical assays and cellular behavior in comprehending the relationship between chemical-based, human plasma oxidation, and CAA.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10965461 | PMC |
| http://dx.doi.org/10.1016/j.crfs.2024.100714 | DOI Listing |
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