AI Article Synopsis
- mRNA plays a crucial role in the development and growth of cells, but it's challenging to detect it in live cells.
- A new strategy was created using split-aptamers and split fluorescent proteins to form dual-color "turn-on" probes that specifically target complementary mRNA sequences.
- This approach, called sAPiFC, offers improved selectivity, stability, and the ability to target different types of mRNAs in live-cell imaging.
Article Abstract
Messenger RNA (mRNA) is an essential component of cell development and growth. However, the detection of endogenous mRNA in living cells is currently limited. To address this issue, we have developed a novel strategy that comprises split-aptamer and split fluorescent protein dual-color, "turn-on" probes that specifically target mRNA with complementary sequences. Our split-aptamer and split-protein-initiated fluorescence complementation (sAPiFC) approach for live-cell imaging has demonstrated selectivity, stability, and capability for targeting various mRNAs.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10964932 | PMC |
| http://dx.doi.org/10.1039/d3ra08475b | DOI Listing |
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