Elucidating the intricate interactions between viral pathogens and host cellular machinery during infection is paramount for understanding pathogenic mechanisms and identifying potential therapeutic targets. The RNA modification N-methyladenosine (mA) has emerged as a significant factor influencing the trajectory of viral infections. Hence, the precise and quantitative mapping of mA modifications in both host and viral RNA is pivotal to understanding its role during viral infection. With the rapid advancement of sequencing technologies, scientists are able to detect mA modifications with various quantitative, high-resolution, transcriptome approaches. These technological strides have reignited research interest in mA, underscoring its significance and prompting a deeper investigation into its dynamics during viral infections. This review provides a comprehensive overview of the historical evolution of mA epitranscriptome sequencing technologies, highlights the latest developments in transcriptome-wide mA mapping, and emphasizes the innovative technologies for detecting mA modification. We further discuss the implications of these technologies for future research into the role of mA in viral infections.
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| http://dx.doi.org/10.1186/s11658-024-00564-y | DOI Listing |
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