This study aimed to answer the question formulated according to the PICO strategy: 'Which essential oils show antimicrobial activity against biofilms formed on dental acrylic resin?' composed by population (dental acrylic resin), intervention (application of essential oils), comparison (denture cleansers, antifungal drugs, chlorhexidine, and oral mouthwashes), and outcome (antibiofilm activity). experimental studies evaluating the activity of EOs on biofilm formed on acrylic resin were included. PRISMA guidelines were followed, and the search was performed in the PubMed, Science Direct, Embase, and Lilacs databases and in the gray literature using Google Scholar and ProQuest in December 2023. A manual search of the reference lists of the included primary studies was performed. Of the 1467 articles identified, 37 were selected for full-text reading and 12 were included. Twelve EOs were evaluated, of which 11 showed activity against spp., 3 against , and 1 against . The EOs of and showed higher action than chlorhexidine, higher than Listerine, higher than nystatin, and higher than fluconazole and nystatin. However, chlorhexidine was more effective than and , sodium hypochlorite was more effective than , nystatin was more effective than , Amphotericin B more effective than and . In conclusion, the EOs of and showed antimicrobial activity to reduce biofilm on dental acrylic resin.
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