Our objective was to determine whether the twice-weekly screening of high-risk hematology patients by Mucorales qPCR on serum affects the prognosis of mucormycosis. Results from all serum Mucorales qPCR tests performed on patients from the hematology unit from January 2017 to December 2022 were analyzed. Patients with positive results were classified as having proven, probable or 'PCR-only' mucormycosis. One-month mortality for the local cohort was compared with that of a national cohort of cases of mucormycosis collected by the French surveillance network for invasive fungal disease ('Réseau de surveillances des infections fongiques invasives en France' (RESSIF)) from 2012 to 2018. From 2017 to 2022, 7825 serum Mucorales qPCR tests were performed for patients from the hematology unit; 107 patients with at least one positive Mucorales qPCR (164 positive samples) were identified. Sixty patients (70 positive samples, median Cq = 40) had no radiological criteria for mucormycosis and were considered not to have invasive fungal disease (70/7825, 0.9% false positives). It was not possible to classify disease status for six patients (12 positive samples, median Cq = 38). Forty-one patients (82 positive samples, median Cq = 35) had a final diagnosis of mucormycosis. In comparison with the RESSIF cohort, the local cohort was independently associated with a 48% lower one-month all-cause mortality rate (age-, sex-, and primary disease-adjusted hazard ratio = 0.52; 95% confidence interval: 0.29-0.94; P 0.03). Proactive screening for invasive mold diseases in high-risk hematology patients, including twice-weekly Mucorales qPCR on serum, was associated with mucormycosis higher survival.
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http://dx.doi.org/10.1093/mmy/myae030 | DOI Listing |
J Clin Microbiol
December 2024
Chrono-environnement UMR6249, CNRS, University of Franche-Comté, Besançon, Bourgogne-Franche-Comté, France.
World J Microbiol Biotechnol
November 2024
Colin Ratledge Center for Microbial Lipids, College of Agricultural Engineering and Food Science, Shandong University of Technology, Zibo, 255000, China.
Astaxanthin is a bioactive natural pigment with antioxidant properties. It has extensive applications within the industrial sector as well as in human and animal health. Mucor circinelloides is a zygomycete fungus that accumulates β-carotene as the main carotenoid compound.
View Article and Find Full Text PDFAnn Med
December 2024
Center of Infectious Disease, West China Hospital, Sichuan University, Chengdu, Sichuan, China.
Open Forum Infect Dis
August 2024
Department of Infectious Diseases, Hôpital Saint-Louis, Assistance Publique-Hôpitaux de Paris, Paris, France.
Microbiol Spectr
September 2024
School of Public Health, Anhui Medical University, Anhui, China.
Unlabelled: Filamentous fungi present significant health hazards to immunocompromised individuals globally; however, the prompt and precise identification of them during infection remains challenging. In this study, a TaqMan probe-based multiplex real-time PCR (M-qPCR) assay was developed to detect simultaneously the target genes of four important pathogenic filamentous fungi: gene of gene of spp, mitochondrial gene of Mucorales, and gene of . In this M-qPCR assay, the limit of detection (LoD) to all four kinds of fungi was 100 copies and the correlation coefficients (R) were above 0.
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