AI Article Synopsis
Article Abstract
T cells coordinate intercellular communication through the meticulous regulation of cytokine secretion. Direct visualization of vesicular transport and intracellular distribution of cytokines provides valuable insights into the temporal and spatial mechanisms involved in regulation. Employing Jurkat E6-1 T cells and interleukin-2 (IL-2) as a model system, we investigated vesicular dynamics using single-particle tracking and the nanoscale distribution of intracellular IL-2 in fixed T cells using superresolution microscopy. Live-cell imaging revealed that in vitro activation resulted in increased vesicular dynamics. Direct stochastic optical reconstruction microscopy and 3D structured illumination microscopy revealed nanoscale clustering of IL-2. In vitro activation correlated with spatial accumulation of IL-2 nanoclusters into more pronounced and elongated clusters. These observations provide visual evidence that accelerated vesicular transport and spatial concatenation of IL-2 clusters at the nanoscale may constitute a potential mechanism for modulating cytokine release by Jurkat T cells.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11331045 | PMC |
| http://dx.doi.org/10.1016/j.bpj.2024.03.029 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
The impact of surface charge on the interaction of cholesterol-free fusogenic liposomes with planktonic microbial cells and biofilms.
Int J Pharm
December 2024
Department of Life Sciences and Health, Oslo Metropolitan University (OsloMet), Oslo, Norway.
This study focused on the development of cholesterol-free fusogenic liposomes with different surface charge with the aim of improving biofilm penetration. In vitro assessments of the liposomes included physical stability, biocompatibility, fusion with microbial cells, and the ability to penetrate established biofilms. Using dynamic light scattering, cholesterol-free, fusogenic liposomes were found to be < 200 nm in size with small size distribution (PDI < 0.
View Article and Find Full Text PDFNear full-length genome sequence of a vesicular stomatitis New Jersey virus isolate collected from a naturally infected cow in the endemic state of Chiapas, Mexico.
Microbiol Resour Announc
December 2024
Foreign Animal Disease Research Unit, Plum Island Animal Disease Center, Agricultural Research Service, U.S. Department of Agriculture, Greenport, New York, USA.
Here, we report the near full-length genome sequence of a isolate obtained from a naturally infected cow () in the state of Chiapas, Mexico. This sequence will support future efforts to improve our understanding of the evolutionary dynamics of this pathogen in endemic regions of Mexico.
View Article and Find Full Text PDFNon-lytic spread of poliovirus requires the nonstructural protein 3CD.
mBio
December 2024
Department of Microbiology and Immunology, The University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA.
Unlabelled: Non-enveloped viruses like poliovirus (PV) have evolved the capacity to spread by non-lytic mechanisms. For PV, this mechanism exploits the host secretory autophagy pathway. Virions are selectively incorporated into autophagosomes, double-membrane vesicles that travel to the plasma membrane, fuse, and release single-membrane vesicles containing virions.
View Article and Find Full Text PDFBiological and computational assessments of thiazole derivative-reinforced bile salt enriched nano carriers: a new gate in targeting SARS-CoV-2 spike protein.
RSC Adv
December 2024
Department of Pharmaceutical Chemistry, College of Pharmacy, The University of Mashreq Baghdad 10023 Iraq.
There is merit in investigating novel therapeutic molecules that hit vital targets during the viral infection cycle disrupting the interaction between SARS-CoV-2's spike glycoprotein and the host's angiotensin converting enzyme 2 (ACE2) receptor, potentially offering new avenues for treatment. Accordingly, lipid-based vesicular systems like liposomes or niosomes are frequently utilized to overcome these hurdles. Thus, chemically synthesized compounds were encapsulated within PEGylated bilosomes (PBs) to improve their solubility and intestinal permeability, thereby enhancing their anti-SARS-CoV-2 effectiveness.
View Article and Find Full Text PDFSNARE mimicry by the CD225 domain of IFITM3 enables regulation of homotypic late endosome fusion.
EMBO J
December 2024
HIV Dynamics and Replication Program, Center for Cancer Research, National Cancer Institute, Frederick, MD, USA.
Article Synopsis
- * Research reveals that the CD225 domain harbors a SNARE-like motif, enabling interactions with SNARE proteins, which are essential for membrane fusion; this is particularly important in diseases linked to mutations in these regions, such as neurological disorders.
- * One member, IFITM3, is shown to interact with SNARE proteins to protect against influenza A virus by disrupting SNARE complex assembly and enhancing endosomal cargo movement to lysosomes, suggesting a key role for SNARE modulation in the diverse functions of CD225
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!