Rice is one of the crops cultivated in Malaysia, and it is the main diet for most of the population. In this study, dispersive liquid-liquid micro-extraction (DLLME) and liquid chromatography-tandem mass spectrometry (LC-MS/MS) were used to develop, optimise and validate a reliable, easy-to-use and quick approach to detect aflatoxin B1 (AFB1), aflatoxin B2 (AFB2), aflatoxin G1 (AFG1) and aflatoxin G2 (AFG2). The extraction recoveries in DLLME were enhanced by the addition of 5% salt, utilising chloroform as the extraction solvent and acetonitrile as the dispersive solvent. The DLLME parameters - the extraction solvent volume, salt concentration and dispersive solvent volume were optimised with Box-Behnken design (BBD) and response surface methodology (RSM). Under optimised experimental conditions, excellent linearity was obtained with a limit of detection (LOD) ranging from 0.125 to 0.25 ng g, a limit of quantitation (LOQ) ranging from 0.25 to 0.3 ng g and a correlation value () of 0.990. The matrix effects were between -11.1% and 19.9%, and recoveries ranged from 87.4% to 117.3%. The optimised and validated method was used effectively to assess aflatoxins contamination in 20 commercial rice samples collected from local supermarkets in Penang, Malaysia. AFB1 was detected at 0.41-0.43 ng g in two rice samples, below the regulatory limit.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1080/19440049.2024.2329614 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Regulatory mechanisms and applications of biofilms in the food industry.
Front Microbiol
January 2025
School of Biotechnology and Food Engineering, Suzhou University, Suzhou, China.
is widely recognized for its probiotic benefits and has been widely used in food production. While biofilms are typically associated with pathogenic bacteria, they also served as a self-protective mechanism formed by microorganisms in an adverse environments. In recent years, relevant studies have revealed the excellent characteristics of biofilms, offering new insights into their potential applications in the food industry.
View Article and Find Full Text PDFMedication reconciliation: impact of an educational intervention on the knowledge, attitude and practices of healthcare professionals - a prospective quasi-experimental study in a Saudi referral hospital.
J Health Popul Nutr
January 2025
Al Wafa Dental Center, Unayzah, Al Qassim, Saudi Arabia.
Background: Medication reconciliation has been acknowledged as a key intervention against medication errors. More than half of the medication errors that happen during care transitions are caused by unjustified medication discrepancies and up to one-third of these mistakes may be harmful. The study aimed to evaluate the knowledge, attitude and practices (KAP) of health care providers in on medication reconciliation process, pre and post educational intervention.
View Article and Find Full Text PDFGlobal landscape of Vibrio parahaemolyticus research: a bibliometric analysis.
World J Microbiol Biotechnol
January 2025
Institute of Climate Adaptation and Marine Biotechnology (ICAMB), Universiti Malaysia Terengganu, Kuala Nerus, Terengganu, 21030, Malaysia.
Vibrio parahaemolyticus poses a notable threat to marine ecosystems and can cause infections and disease outbreaks in seafood species, which can affect humans upon consumption. The global impacts of such infections and outbreaks on human and animal health led to a growing number of studies from various countries discussing the prevention, control, treatment, and overall implications of V. parahaemolyticus.
View Article and Find Full Text PDFComparison Between Molecular Dynamics Potentials for Simulation of Graphene-Based Nanomaterials for Biomedical Applications.
Drug Dev Ind Pharm
January 2025
Department of Pharmaceutical Analysis and Medicinal Chemistry, Faculty of Pharmacy, Universitas Padjadjaran, Jl. Raya Bandung Sumedang KM 21, Sumedang 45363, Indonesia.
Objective: This article provides a substantial review of recent research and comparison on molecular dynamics potentials to determine which are most suitable for simulating the phenomena in graphene-based nanomaterials (GBNs).
Significance: GBNs gain significant attention due to their remarkable properties and potential applications, notably in nanomedicine. However, the physical and chemical characteristics toward macromolecules that justify their nanomedical applications are not yet fully understood.
A prospective randomized controlled trial comparing biphasic cartilage repair implant with microfracture in small chondral lesions of knee: findings at five-year-follow-up.
J Orthop Surg Res
January 2025
Department of Orthopedics, Shuang Ho Hospital, Taipei Medical University, 291, Zhongzheng Rd, Zhonghe Dist, New Taipei City, 23561, Taiwan.
Background: Full-thickness cartilage defects have a significant impact on the function of joints in young adults, and the treatment of cartilage defects has been a challenge, as cartilage tissue is an avascular tissue. This study aimed to compare the clinical and radiological outcomes of Biphasic Cartilage Repair Implant (BiCRI) and microfracture treatments for knee cartilage defects.
Methods: This randomized controlled clinical trial enrolled patients with symptomatic knee chondral lesions smaller than 3 cm.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!