AI Article Synopsis

  • * In fission yeast, a mutant form of the ufd1+ gene shows sensitivity to high levels of CENP-A (Cnp1), leading to its mislocalization and abnormal accumulation at the centromere.
  • * The study suggests that the interaction between Cdc48 ATPase and its cofactor Ufd1 helps regulate Cnp1 levels at centromeres, which might be essential for centromere function during cell development and response to stress.

Article Abstract

CENP-A determines the identity of the centromere. Because the position and size of the centromere and its number per chromosome must be maintained, the distribution of CENP-A is strictly regulated. In this study, we have aimed to understand mechanisms to regulate the distribution of CENP-A (Cnp1SP) in fission yeast. A mutant of the ufd1+ gene (ufd1-73) encoding a cofactor of Cdc48 ATPase is sensitive to Cnp1 expressed at a high level and allows mislocalization of Cnp1. The level of Cnp1 in centromeric chromatin is increased in the ufd1-73 mutant even when Cnp1 is expressed at a normal level. A preexisting mutant of the cdc48+ gene (cdc48-353) phenocopies the ufd1-73 mutant. We have also shown that Cdc48 and Ufd1 proteins interact physically with centromeric chromatin. Finally, Cdc48 ATPase with Ufd1 artificially recruited to the centromere of a mini-chromosome (Ch16) induce a loss of Cnp1 from Ch16, leading to an increased rate of chromosome loss. It appears that Cdc48 ATPase, together with its cofactor Ufd1 remove excess Cnp1 from chromatin, likely in a direct manner. This mechanism may play a role in centromere disassembly, a process to eliminate Cnp1 to inactivate the kinetochore function during development, differentiation, and stress response.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11033524PMC
http://dx.doi.org/10.1242/bio.060287DOI Listing

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