Although pigs are naturally susceptible to Reston virus and experimentally to Ebola virus (EBOV), their role in Orthoebolavirus ecology remains unknown. We tested 888 serum samples collected from pigs in Guinea during 2017-2019 (between the 2013-16 epidemic and its resurgence in 2021) by indirect ELISA against the EBOV nucleoprotein. We identified 2 hotspots of possible pig exposure by IgG titer levels: the northern coast had 48.7% of positive serum samples (37/76), and Forest Guinea, bordering Sierra Leone and Liberia, where the virus emerged and reemerged, had 50% of positive serum samples (98/196). The multitarget Luminex approach confirms ELISA results against Ebola nucleoprotein and highlights cross-reactivities to glycoprotein of EBOV, Reston virus, and Bundibugyo virus. Those results are consistent with previous observations of the circulation of Orthoebolavirus species in pig farming regions in Sierra Leone and Ghana, suggesting potential risk for Ebola virus disease in humans, especially in Forest Guinea.
tRNA-derived stress-induced RNAs (tiRNAs) are a new class of small non-coding RNA that have emerged as important regulators of cellular stress responses. tiRNAs are derived from specific tRNA cleavage by the stress-induced ribonuclease angiogenin (ANG). Loss-of-function mutations in the ANG gene are linked to amyotrophic lateral sclerosis (ALS), and elevated levels of specific tiRNAs were recently identified in ALS patient serum samples.
Non-invasive glucose monitoring is crucial for diabetes management. This study explores the use of photoacoustic (PA) signals based on optical rotation estimation at multiple depths for detection of glucose concentrations. Experiments were performed with glucose samples mixed in bovine serum albumin with different polarization incidences-vertical (V), 45° linear (P), and right circular (R) polarization.
Nanoscale aggregates play a key role in the pathogenesis of neurodegenerative disorders such as Alzheimer's and Parkinson's disease. However, quantifying these aggregates in complex biological samples, such as biofluids and postmortem brain tissue, has been challenging due to their low concentration and small size, necessitating the development of methods with high sensitivity and specificity. Here, we have developed ultrasensitive assays utilizing the Quanterix Simoa platform to detect α-synuclein, β-amyloid and tau aggregates, including those with common posttranslational modifications such as truncation of α-synuclein and AT8 phosphorylation of tau aggregates.
The optical detection of arsenic (As) in human biological fluids and environmental water samples is presented using alpha-cyclodextrin-modified silver nanoparticles (α/CyD-AgNPs) at the trace level. This method is based on the measurement of a red shift of the LSPR band of α/CyD-AgNPs in the region of 200-800 nm. The color of α/CyD-AgNPs was changed from yellow to colorless by the addition of As(iii).
Background And Objective: Contagious caprine pleuropneumonia (CCPP) is a highly contagious mycoplasmal respiratory disease primarily affecting goats and sheep caused by subsp. (Mccp). So far, there is no available information on either the serological or molecular identification of Mccp in Bangladesh.
