AI Article Synopsis
- Base excision is an important DNA repair process, with CpendoIV being the primary endonuclease in Chlamydia pneumoniae, showing potential for anti-chlamydial drug development due to its proofreading capabilities.
- Molecular docking studies analyzed CpendoIV's structure and binding with DNA, revealing important protein surface properties and comparison to E. coli EndoIV.
- Successful cloning, expression, and purification of CpendoIV were achieved, with crystallization conducted to further understand its role in the base excision repair process.
Article Abstract
Base excision is a crucial DNA repair process mediated by endonuclease IV in nucleotide excision. In Chlamydia pneumoniae, CpendoIV is the exclusive AP endonuclease IV, exhibiting DNA replication error-proofreading capabilities, making it a promising target for anti-chlamydial drug development. Predicting the structure of CpendoIV, molecular docking with DNA was performed, analyzing complex binding sites and protein surface electrostatic potential. Comparative structural studies were conducted with E. coli EndoIV and DNA complex containing AP sites.CpendoIV was cloned, expressed in E. coli, and purified via Ni-NTA chelation and size-exclusion chromatography. Low NaCl concentrations induced aggregation during purification, while high concentrations enhanced purity.CpendoIV recognizes and cleaving AP sites on dsDNA, and Zn influences the activity. Crystallization was achieved under 8% (v/v) Tacsimate pH 5.2, 25% (w/v) polyethylene glycol 3350, and 1.91 Å resolution X-ray diffraction data was obtained at 100 K. This research is significant for provides a deeper understanding of CpendoIV involvement in the base excision repair process, offering insights into Chlamydia pneumoniae.
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| http://dx.doi.org/10.1016/j.pep.2024.106476 | DOI Listing |
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