Background: Hearing loss is a common sensorineural dysfunction with a high incidence in China. Although genetic factors are important causes of hearing loss, hearing-related gene detection has not been widely adopted in China.
Objective: Establishing a rapid and efficient method to simultaneously detect hotspot hearing loss gene mutations.
Methods: A reverse dot blot assay combined with a flow-through hybridization technique was developed for the simultaneous detection of 13 hotspot mutations of 4 hearing loss-related genes including and the mitochondrial gene . This method involved PCR amplification systems and a hybridization platform.
Results: The technique can detect 13 hotspot mutations of 4 hearing loss-related genes. And a total of 213 blood samples were used to evaluate the availability of this method.
Discussion: Our reverse dot blot assay was a simple, rapid, accurate, and cost-effective method to identify hotspot mutations of 4 hearing loss-related genes in a Chinese population.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10953844 | PMC |
| http://dx.doi.org/10.2478/abm-2024-0003 | DOI Listing |
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