miR-317-3p and miR-283-5p Play a Crucial Role in Regulating the Resistance to Indoxacarb in by Targeting .

is primarily controlled through chemical insecticides. Our RNA-seq data highlight the overexpression of in indoxacarb-resistant . However, the exact role of in indoxacarb resistance and its regulatory mechanisms remains elusive. Therefore, we investigated the functional role of in and explored the underlying post-transcriptional regulatory mechanisms. was highly overexpressed (27.6-fold) in the indoxacarb-resistant strain, and silencing significantly increases the susceptibility of to indoxacarb, increasing mortality by 27.3%. miR-317-3p and miR-283-5p can bind to the 3'UTR of , and the targeting relationship was confirmed by dual-luciferase reporter assays. Injecting miR-317-3p and miR-283-5p agomirs reduces levels by 64.8 and 42.3%, respectively, resulting in an increased susceptibility of to indoxacarb. Conversely, the administration of miR-317-3p and miR-283-5pantagomirs increases expression and reduces larval susceptibility to indoxacarb. These findings demonstrate that miR-317-3p and miR-283-5p contribute to indoxacarb resistance in by regulating the overexpression of .