The Gas family of β-1,3-glucanosyltransferases modify the cell wall by elongating β-1,3-glucan chains. While cells are inviable under standard laboratory growth conditions, they are viable in the presence of an osmotic stabilizer. Even under these conditions however, cells are slow-growing and display cell separation and morphology defects. Here, we isolated and characterized two temperature-sensitive alleles. Our data support that Gas1 is the primary β-1,3-glucanosyltransferase important for cell separation and cell viability and provide useful tools for further analysis of cell wall formation.
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| http://dx.doi.org/10.17912/micropub.biology.001144 | DOI Listing |
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