Uric acid is an adequate and endogenous probe for identifying reactive oxygen or nitrogen species generated because its oxidation products are specific to reacted reactive oxygen or nitrogen species. Recently, we identified 5--carboxyimino-6--chloroaminopyrimidine-2,4(3)-dione as a hypochlorite-specific oxidation product. 5--carboxyimino-6--chloroaminopyrimidine-2,4(3)-dione was anticipated to be a biomarker for hypochlorite production . However, while it was stable in aqueous solution at weak acidic and alkaline pH (6.0-8.0), it was unstable in human plasma. In this study, we found that 5--carboxyimino-6--chloroaminopyrimidine-2,4(3)-dione rapidly reacted with thiol compounds such as cysteine and glutathione to yield 5--carboxyimino-6-aminopyrimidine-2,4(3)-dione, which was stable in human plasma unlike 5--carboxyimino-6--chloroaminopyrimidine-2,4(3)-dione. 5--carboxyimino-6-aminopyrimidine-2,4(3)-dione was produced upon uric acid degradation during myeloperoxidase-induced uric acid oxidation and lipopolysaccharide-induced pseudo-inflammation in collected 2,4(3)-dione has potential as a marker for hypochlorite production .
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10948346 | PMC |
| http://dx.doi.org/10.3164/jcbn.23-67 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Enzyme cascade nanozyme based colorimetric sensor for detection of uric acid as a biomarker of hyperuricemia.
Mikrochim Acta
January 2025
Key Laboratory of Organic Integrated Circuit, Ministry of Education & Tianjin Key Laboratory of Molecular Optoelectronic Sciences, Department of Chemistry, School of Science, Tianjin University, Tianjin, 300072, China.
A Cr-doped VO nanobelt (Cr/VO) with remarkable peroxidase-like activity was synthesized and coupled with uricase to catalyze the cascade reaction for detection of uric acid. Notably, the affinity of Cr/VO for 3,3',5,5'-tetramethylbenzidine dihydrochloride hydrate (TMB) and hydrogen peroxide (HO) is tenfold and 20-fold higher, respectively, than that of horseradish peroxidase (HRP). The Cr/VO exhibits highly reactive and stable peroxidase activity at temperatures of 20-60 ℃.
View Article and Find Full Text PDFExcess Weight Loss at 6 Months Following Laparoscopic Sleeve Gastrectomy Correlates with the Remission of Hyperuricemia.
Obes Surg
January 2025
Center for Obesity and Hernia Surgery, Department of General Surgery, Huashan Hospital, Fudan University, Shanghai, 200040, China.
Background: Hyperuricemia is a metabolic disorder associated with obesity. Many studies have reported the effect of bariatric surgery on the decrease of serum uric acid level in patients with hyperuricemia. However, since the update of diagnostic criteria of hyperuricemia, the correlation between preoperative body mass index, postoperative weight changes, and the remission of hyperuricemia in patients with obesity after sleeve gastrectomy requires consensus.
View Article and Find Full Text PDFAssociation of homocysteine and uric acid with type 2 diabetes mellitus: a case-control study.
Sci Rep
January 2025
School of Public Health, Ningxia Medical University, Yinchuan, 75004, China.
The aim of this study was to examine the association between homocysteine (Hcy), uric acid (UA) and type 2 diabetes mellitus (T2DM), and to explore whether there was an interaction between Hcy and UA in the development of T2DM. A total of 1250 diabetic patients and 1250 non-diabetic controls were included in this case-control study. Binary logistic regression and interaction analysis were used to evaluate the association between Hcy, UA, and T2DM, and the combined effects of Hcy and UA on T2DM, respectively.
View Article and Find Full Text PDFThe influence of serum uric acid on coronary atherosclerosis plaque phenotypes by computed tomography angiography: The missing link?
Nutr Metab Cardiovasc Dis
December 2024
Department of Radiology, Innsbruck Medical University, Innsbruck, Austria. Electronic address:
Background And Aims: The interaction of serum uric acid (SUA) with atherogenesis is incompletely understood. Aim of our study was to investigate the association of SUA levels with coronary plaque composition including high-risk-plaque (HRP) features by coronary computed tomography angiography (CTA) and for the prediction of major adverse cardiac events (MACE).
Methods And Results: 1242 patients (age 66.
Effects of 3-month liraglutide treatment on oxidative stress and inflammation in type 2 diabetes patients with different urinary albumin-to-creatinine ratio categories.
Medicine (Baltimore)
November 2024
Department of Endocrinology of Chongqing Red Cross Hospital (People's Hospital of Jiangbei District), Chongqing, China.
This study evaluates the effects of liraglutide on albuminuria, oxidative stress, and inflammation in type 2 diabetes (T2D) patients with different urinary albumin-to-creatinine ratio (UACR) categories. We enrolled 107 patients with T2D who were initiating liraglutide for glycemic control. Patients were categorized into 3 groups: group I (UACR < 30 mg/g); group II (30 mg/g ≤ UACR ≤ 300 mg/g); group III (UACR > 300 mg/g).
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!