AI Article Synopsis

  • The study focuses on an opportunistic pathogen that thrives in harsh environments and often leads to outbreaks in neonatal intensive care units (NICUs), primarily caused by a specific clone, referred to as the "Infectious clone."
  • It introduces a new method called hypervariable-locus melting typing (HLMT) that allows rapid detection and typing of this pathogen without the need for cultivation, achieving high accuracy with 97% specificity and 96% sensitivity compared to traditional whole genome sequencing (WGS).
  • The HLMT protocol is advantageous for large-scale environmental and clinical screenings, offering cost and time efficiency that is particularly beneficial in low- to middle-income countries.

Article Abstract

is an opportunistic pathogen that survives in inhospitable environments causing large outbreaks, particularly in neonatal intensive care units (NICUs). Genomic studies revealed that most nosocomial infections are caused by a specific clone (here "Infectious clone"). Whole genome sequencing (WGS) is the only portable method able to identify this clone, but it requires days to obtain results. We present a cultivation-free hypervariable-locus melting typing (HLMT) protocol for the fast detection and typing of , with 100% detection capability on mixed samples and a limit of detection that can reach the 10 genome copies. The protocol was able to identify the infectious clone with 97% specificity and 96% sensitivity when compared to WGS, yielding typing results portable among laboratories. The protocol is a cost and time saving method for detection and typing for large environmental/clinical surveillance screenings, also in low-middle income countries.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10952028PMC
http://dx.doi.org/10.1016/j.isci.2024.109402DOI Listing

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