Neoantigen delivery using extracellular vesicles (EVs) has gained extensive interest in recent years. EVs derived from tumour cells or immune cells have been used to deliver tumour antigens or antitumor stimulation signals. However, potential DNA contamination from the host cell and the cost of large-scale EV production hinder their therapeutic applications in clinical settings. Here, we develop an antigen delivery platform for cancer vaccines from red blood cell-derived EVs (RBCEVs) targeting splenic DEC-205 dendritic cells (DCs) to boost the antitumor effect. By loading ovalbumin (OVA) protein onto RBCEVs and delivering the protein to DCs, we were able to stimulate and present antigenic OVA peptide onto major histocompatibility complex (MHC) class I, subsequently priming activated antigen-reactive T cells. Importantly, targeted delivery of OVA using RBCEVs engineered with anti-DEC-205 antibody robustly enhanced antigen presentation of DCs and T cell activation. This platform is potentially useful for producing personalised cancer vaccines in clinical settings.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11216926 | PMC |
| http://dx.doi.org/10.1111/cpr.13622 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Delivery of genetic medicines for muscular dystrophies.
Cell Rep Med
December 2024
Laboratory of Precision Nanomedicine, The Shmunis School of Biomedicine and Cancer Research, George S. Wise Faculty of Life Sciences, Tel Aviv University, Tel-Aviv, Israel; Department of Materials Sciences and Engineering, Iby and Aladar Fleischman Faculty of Engineering, Tel Aviv University, Tel Aviv, Israel; Center for Nanoscience and Nanotechnology, Tel Aviv University, Tel Aviv, Israel; Cancer Biology Research Center, Tel Aviv University, Tel Aviv, Israel. Electronic address:
Muscular dystrophies are a group of heterogenic disorders characterized by progressive muscle weakness, the most common of them being Duchenne muscular dystrophy (DMD). Muscular dystrophies are caused by mutations in over 50 distinct genes, and many of them are caused by different genetic mechanisms. Currently, none of these diseases have a cure.
View Article and Find Full Text PDFProspects of bovine milk small extracellular vesicles in veterinary medicine.
Res Vet Sci
January 2025
Laboratory of Food and Environmental Hygiene, Joint Department of Veterinary Medicine, Gifu University, Gifu, Japan; Education and Research Center for Food Animal Health, Gifu University (GeFAH), Gifu, Japan; Joint Graduate School of Veterinary Sciences, Gifu University, Gifu, Japan. Electronic address:
Extracellular vesicles (EV), including exosomes or small EV (sEV) derived from biological fluids, such as milk, have garnered increasing interest in veterinary medicine because of their role in the pathophysiology and understanding of the disease status of the host. Bovine milk serves as a rich source of sEV, containing diverse cargoes of nucleic acids, proteins, and lipids, which play a critical role in intercellular communication and regulation of host status. Although it is more difficult to isolate and purify sEV from bovine milk than from human breast milk, challenges persist in enabling the enrichment and analysis of sEV populations, facilitating the elucidation of their functional roles and prognostic potential in cattle diseases.
View Article and Find Full Text PDFExosomes Derived from Human Adipose Mesenchymal Stem Cells act as a therapeutic target for Oral Submucous Fibrosis.
J Stomatol Oral Maxillofac Surg
January 2025
Centre of Molecular Medicine and Diagnostics (COMManD), Department of Biochemistry, Saveetha Dental College & Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, 600077 Tamil Nadu, India. Electronic address:
Oral submucosal fibrosis is a highly malignant oral condition that necessitates the use of sophisticated therapeutic procedures. OSF is a multifactorial precancerous condition induced by areca nut chewing, deficiencies in vitamins and trace minerals, immunological aspects, and hereditary factors. Adipose tissue-derived mesenchymal stem cells possess the capability for multidirectional activation and are extensively distributed throughout the body.
View Article and Find Full Text PDFExosomes for Aesthetic Dermatology: A Comprehensive Literature Review and Update.
J Cosmet Dermatol
January 2025
Clinical Research Center of the Carolinas, Charleston, South Carolina, USA.
Background: Exosomes are nanoscale vesicles derived from various cell types and tissues that have many potential applications, generating great interest from researchers. One particularly intriguing application of exosomes is their use as a direct therapeutic for aesthetic indications. Several studies and case reports have explored the impact of exosomes for numerous cosmetic concerns but a consensus on the outcomes of these studies has not been established.
View Article and Find Full Text PDFA noninvasive urinary microRNA-based assay for the detection of pancreatic cancer from early to late stages: a case control study.
EClinicalMedicine
December 2024
Department of Pathology and Genetics, Laboratory of Cancer Medical Science, Hokuto Hospital, Obihiro, Hokkaido, Japan.
Background: Pancreatic cancer is highly aggressive and has a low survival rate primarily due to late-stage diagnosis and the lack of effective early detection methods. We introduce here a novel, noninvasive urinary extracellular vesicle miRNA-based assay for the detection of pancreatic cancer from early to late stages.
Methods: From September 2019 to July 2023, Urine samples were collected from patients with pancreatic cancer (n = 153) from five distinct sites (Hokuto Hospital, Kawasaki Medical School Hospital, National Cancer Center Hospital, Kagoshima University Hospital, and Kumagaya General Hospital) and non-cancer participants (n = 309) from two separate sites (Hokuto Hospital and Omiya City Clinic).
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!