An enrichment of sulfidic sediments from Zodletone spring was sequenced as a metagenome. Draft genomes representing Cloacimonadota, Deltabacterota, Firmicutes, and Patescibacteria were binned and annotated and will aid functional genomics and cultivation efforts.
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http://dx.doi.org/10.1128/mra.00114-24 | DOI Listing |
Int J Syst Evol Microbiol
August 2024
School of Biological Sciences, University of Oklahoma, Norman, Oklahoma, USA.
Four methane-oxidizing bacteria, designated as strains WSC-6, WSC-7, SURF-1, and SURF-2, were isolated from Saddle Mountain Creek in southwestern Oklahoma, USA, and the Sanford Underground Research Facility (SURF) in Lead, South Dakota, USA. The strains were Gram-negative, motile, short rods that possessed intracytoplasmic membranes characteristic of type I methanotrophs. All four strains were oxidase-negative and weakly catalase-positive.
View Article and Find Full Text PDFMicrobiol Resour Announc
April 2024
Department of Microbiology, University of Tennessee Knoxville, Knoxville, Tennessee, USA.
An enrichment of sulfidic sediments from Zodletone spring was sequenced as a metagenome. Draft genomes representing Cloacimonadota, Deltabacterota, Firmicutes, and Patescibacteria were binned and annotated and will aid functional genomics and cultivation efforts.
View Article and Find Full Text PDFMicrobiol Resour Announc
May 2023
Department of Microbiology, University of Tennessee Knoxville, Knoxville, Tennessee, USA.
sp. strain ZS1 is an obligate anaerobic, sulfate-reducing member of the from Zodletone Spring, an anoxic sulfide-rich spring in southwestern Oklahoma. Its complete genome was sequenced using a combination of Illumina and Oxford Nanopore platforms and encodes 3,364 proteins and 81 RNAs on a single chromosome.
View Article and Find Full Text PDFmBio
April 2022
Department of Microbiology and Molecular Genetics, Oklahoma State Universitygrid.65519.3e, Stillwater, Oklahoma, USA.
Life emerged and diversified in the absence of molecular oxygen. The prevailing anoxia and unique sulfur chemistry in the Paleo-, Meso-, and Neoarchean and early Proterozoic eras may have supported microbial communities that differ from those currently thriving on the earth's surface. Zodletone spring in southwestern Oklahoma represents a unique habitat where spatial sampling could substitute for geological eras namely, from the anoxic, surficial light-exposed sediments simulating a preoxygenated earth to overlaid water column where air exposure simulates oxygen intrusion during the Neoproterozoic era.
View Article and Find Full Text PDFAppl Environ Microbiol
November 2021
Department of Microbiology and Molecular Genetics, Oklahoma State Universitygrid.65519.3e, Stillwater, Oklahoma, USA.
Cultured are predominantly aerobic soil inhabitants, characterized by their highly coordinated predation and cellular differentiation capacities. Little is currently known regarding yet-uncultured from anaerobic, nonsoil habitats. We analyzed genomes representing one novel order (o__JAFGXQ01) and one novel family (f__JAFGIB01) in the from an anoxic freshwater spring (Zodletone Spring) in Oklahoma, USA.
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