Objectives: Alpha-lactalbumin, the protein from human and bovine milk has been found to be promising as an alternative of anticancer agent This study was aimed to investigate the effects of lactalbumin enzymatic hydrolysate (LAH) on cell proliferation, migration, and mRNA expression of () on human squamous cell carcinoma (hSCC) cell lines, .
Methods: Tongue (HSC-4 and 7) and pharyngeal (HN-30 and 31) hSCC cell lines were treated with a two-fold dilution of LAH (0.39-100 mg/ml). Cell viability and cell proliferation were examined by MTT assay. Colony forming unit (CFU) was assessed by crystal violet blue staining. Cell migration was investigated by scratch wound healing assay. Gene expression of metastasis-associated was assessed by RT-qPCR. Statistical analyses were evaluated at value = 0.05.
Results: LAH at concentration of 50 and 100 mg/ml exhibited cytotoxicity on hSCC cells. The proliferation and CFU ability of hSCC cells were significantly attenuated after LAH treatment. The mRNA expression of , , and was reduced in HN-30 and HN-31 cells while expression of and was downregulated in HSC-7 cells. Only mRNA level was reduced in HSC-4 cells. However, cell migration of all hSCC cell lines did not alter after LAH treatment.
Conclusion: LAH treatment exhibits inhibitory effects on hSCC cell growth, proliferation and gene expression. Thus, LAH should be the promising alternative agent to develop the prospective anti-cancer drug.
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| http://dx.doi.org/10.1016/j.jobcr.2024.02.011 | DOI Listing |
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