AI Article Synopsis
- Bioburden detection is important for food, water, and pharmaceuticals because it affects public health.
- This study aimed to create a sensitive and accurate method for detecting bioburden on solid surfaces and in water quickly.
- The researchers developed a resazurin-based assay integrated with a portable fluorometer, successfully identifying low levels of contamination in both surfaces and water samples.
Article Abstract
Bioburden detection is crucial for food, water, and biopharmaceutical applications as it can directly impact public health. The objective of this study is to develop and validate an assay and protocol for detecting bioburden on solid surfaces, as well as in water, with high sensitivity and accuracy in a rapid manner. Henceforth, a resazurin-based assay optimized for detecting bioburden has been integrated with a previously developed portable multichannel fluorometer. The microbes were isolated from solid surfaces in different laboratory settings by swabbing technique, and stream water was collected for contamination analysis. Based on the results, the assay and protocol can successfully detect bioburden as low as 20 CFU/cm and 10 CFU/mL present in both surface and water samples, respectively.
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| http://dx.doi.org/10.1002/btpr.3457 | DOI Listing |
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