AI Article Synopsis
- Parallel double-stranded DNA (dsDNA) has useful applications in fields like molecular biology, diagnosis, and therapy, but current methods to study it are limited.
- This research showcases the use of surface-enhanced Raman spectroscopy (SERS) to identify specific Raman bands from three types of parallel dsDNA stabilized by different base pair configurations.
- The new method can distinguish between parallel and antiparallel dsDNA and determine the orientation of the strands, which could greatly improve DNA analysis and the ability to detect different DNA forms.
Article Abstract
The parallel double-stranded DNA (dsDNA) demonstrates potential utility in molecular biology, diagnosis, therapy, and molecular assembly. However, techniques for the characterization of parallel dsDNA are limited. Here, we demonstrate that a series of intensive characteristic Raman bands of three parallel dsDNAs, which are stabilized by reverse Hoogsteen A·A base pairs or hemiprotonated C·C, G·G minor groove edge, Hoogsteen A·A base pairs, or Hoogsteen T·A, C·G base pairs, have been observed by surface-enhanced Raman spectroscopy (SERS) when the gold nanoparticles modified by bromine and magnesium ions (Au BMNPs) were used as substrates. The featured bands can not only accurately discriminate parallel dsDNA from antiparallel one but also identify the strand orientation within dsDNA. The proposed approach will have a significant impact on DNA analysis, especially in the detection and differentiation of various DNA conformations.
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| http://dx.doi.org/10.1021/acs.analchem.3c05356 | DOI Listing |
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