Polybrominated diphenyl ethers (PBDEs) are toxic to organisms with melatonin (MT) providing protection for tissues and cells against these. This study investigates the mechanism of damage of 2,2',4,4'-tetrabromodiphenyl ether (BDE-47) and the cellular protection of MT on grass carp hepatocytes. Grass carp hepatocytes were exposed to 25 μmol/L BDE-47 and/or 40 μmol/L MT for 24 h before testing. Acridine orange/ethidium bromide (AO/EB) double fluorescence staining results showed that BDE-47 could induce cell apoptosis. The expression levels of the endoplasmic reticulum (ER) stress-related genes ire1, atf4, grp78, perk, and chop were also significantly up-regulated (P < 0.01). The levels of the apoptosis-related genes caspase3, bax, and caspase9 were significantly up-regulated (P < 0.0001), while the level of bcl-2 was significantly down-regulated (P < 0.01). Compared with the BDE-47 group, the BDE-47 + MT group showed reduced levels of ER and apoptosis of hepatocytes, while the expression of the ER stress-related genes ire1, atf4, grp78, perk, and chop and the apoptosis-related genes caspase3, bax, and caspase9 were down-regulated (P < 0.05), and the level of bcl-2 was up-regulated (P < 0.01). In conclusion, BDE-47 can activate ER and apoptosis in grass carp hepatocytes, while MT can reduce these responses.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/s11356-024-32856-3 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Evaluation of edible quality and processing suitability of segmented products from silver carp under different thermal processing methods.
Food Res Int
February 2025
College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, PR China; Shanghai Engineering Research Center of Aquatic-Product Processing and Preservation, Shanghai 201306, PR China; Xiweijia Biotechnology Co., Ltd., Yueyang, Hunan 414024, PR China. Electronic address:
This study investigated the edible quality differences in muscle segments of silver carp (Hypophthalmichthys molitrix) and established an evaluation model for processing suitability. The results showed that steamed dorsal meat had the highest levels of total free amino acids, umami amino acids, and total volatile compounds. Fried tail meat exhibited the highest content of sweet amino acids and equivalent umami concentration (EUC) values, which were superior in all fried meat parts compared to those that were steamed.
View Article and Find Full Text PDFThe cardiac response of the goldfish Carassius auratus to environmental hypoxia: from hemodynamics to mitochondria.
Fish Physiol Biochem
January 2025
Department of Biology, Ecology and Earth Science, University of Calabria, Rende, Italy.
Under low O, the heart of Carassius auratus (goldfish) shows an enhanced hemodynamics. This is observed in ex vivo cardiac preparations from animals acclimated to both normoxia and short-term (4 days) moderate hypoxia and perfused for 90 min with a hypoxic medium. Under short-term hypoxia, this is associated with a higher ventricular muscularity and an expanded mitochondrial compartment.
View Article and Find Full Text PDFMultilayer entropy-weighted TOPSIS method and its decision-making in ecological operation during the subsidence period of the Three Gorges Reservoir.
Sci Rep
January 2025
State Key Laboratory of Water Resources Engineering and Management, Wuhan University, Wuhan, 430,072, China.
Coordinating the downstream ecological demand and the power generation demand of hydropower stations is an important task in the operation of reservoirs, and how to evaluate the ecological satisfaction of the scheduling process is a difficult problem that needs to be solved urgently. A multi-objective optimal reservoir scheduling model was constructed to coordinate the spawning flow demand of " Four Major Chinese Carps"; The model takes the maximum power generation and the maximum membership degree of downstream river ecological water demand as the objective functions, and uses the dynamic programming multi-objective solution algorithm based on penalty factors to solve the problem, and obtains the non-inferior solution set in each scenario. The multilayer entropy-weighted TOPSIS method was used to study the non-inferior solution of the multi-objective scheduling model of the Three Gorges Reservoir, and the satisfactory solution ranking of the river flow rise process, ecological flow-related requirements, and power generation water requirements was obtained under the four schemes including 4d ~ 7d, which improved the reliability of the evaluation results and made up for the shortcomings of the traditional TOPSIS method in terms of hierarchy and weight science.
View Article and Find Full Text PDFChromosome-Level Genome Assembly and Whole-Genome Resequencing Revealed Contrasting Population Genetic Differentiation of Black Bream () (Teleostei: Cyprinidae) Allopatric and Sympatric to Its Kin Species.
Ecol Evol
January 2025
State Key Laboratory of Breeding Biotechnology and Sustainable Aquaculture, Institute of Hydrobiology Chinese Academy of Sciences Wuhan China.
The black bream () is an economically important species widely distributed in China, with its geographic populations potentially having undergone differentiations and local adaptations. In this study, we presented a chromosome-level genome assembly of this species and investigated genetic differentiations of its populations that are allopatric (the northern one) and sympatric (the Poyang Lake) to its kin species, the blunt-snout bream (), using whole genome resequencing analysis. The results showed that the genome size of black bream was 1.
View Article and Find Full Text PDFRelations between microplastic contamination and stress biomarkers under two seasonal conditions in wild carps, mullets and flounders.
Mar Environ Res
January 2025
ICBAS - Instituto de Ciências Biomédicas Abel Salazar, Universidade do Porto, Departamento de Estudos de Populações, Laboratório de Ecotoxicologia e Ecologia, (ECOTOX), Rua de Jorge Viterbo Ferreira, 228, 4050-313, Porto, Portugal; CIIMAR / CIMAR-LA - Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Research Team of Aquatic Ecotoxicology and One Health, and Research Team of Contaminant Pathways in Marine Environment, Terminal de Cruzeiros do Porto de Leixões, 4450-208, Matosinhos, Portugal. Electronic address:
Potential effects of microplastics (MP, plastic particles <5 mm) on the levels of multiple stress biomarkers were investigated in wild fish populations of Cyprinus carpio, Mugil cephalus, Platichthys flesus captured in the Minho River estuary located in the Iberian Peninsula. Specimens were collected in March and September 2018, corresponding to the end of winter and summer, respectively. Based on the concentration of MP determined by FT-IR analysis and morphological inspection, fishes from each species were divided into two groups: ≤0.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!