AI Article Synopsis
- Scientists found that the membranes of cells can change to handle stress and challenges, but we don’t know much about how this affects different parts of the cells.
- They developed a new method called MemPrep to study the membranes of yeast cells, especially focusing on the endoplasmic reticulum (ER) and vacuoles.
- The study showed how the lipids (fats) in these membranes change when the cell is stressed, giving important clues to help understand how cells manage problems, like incorrectly folded proteins.
Article Abstract
Biological membranes have a stunning ability to adapt their composition in response to physiological stress and metabolic challenges. Little is known how such perturbations affect individual organelles in eukaryotic cells. Pioneering work has provided insights into the subcellular distribution of lipids in the yeast Saccharomyces cerevisiae, but the composition of the endoplasmic reticulum (ER) membrane, which also crucially regulates lipid metabolism and the unfolded protein response, remains insufficiently characterized. Here, we describe a method for purifying organelle membranes from yeast, MemPrep. We demonstrate the purity of our ER membrane preparations by proteomics, and document the general utility of MemPrep by isolating vacuolar membranes. Quantitative lipidomics establishes the lipid composition of the ER and the vacuolar membrane. Our findings provide a baseline for studying membrane protein biogenesis and have important implications for understanding the role of lipids in regulating the unfolded protein response (UPR). The combined preparative and analytical MemPrep approach uncovers dynamic remodeling of ER membranes in stressed cells and establishes distinct molecular fingerprints of lipid bilayer stress.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11021466 | PMC |
| http://dx.doi.org/10.1038/s44318-024-00063-y | DOI Listing |
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