AI Article Synopsis
- - This study focuses on analyzing B cell receptors (BCRs) to understand both the quantity and quality of memory B cells that respond to specific antigens, particularly in the context of HIV-1 research.
- - Researchers used a method called SCAN to measure how well BCRs neutralize HIV-1 and developed a frequency-potency algorithm to assess B cell frequencies based on their neutralizing strength.
- - The results revealed important insights into the characteristics of HIV-1-specific memory B cells and highlighted the potential of these methodologies for improving vaccine strategies and discovering new monoclonal antibodies.
Article Abstract
Identifying individual functional B cell receptors (BCRs) is common, but two-dimensional analysis of B cell frequency versus BCR potency would delineate both quantity and quality of antigen-specific memory B cells. We efficiently determine quantitative BCR neutralizing activities using a single-cell-derived antibody supernatant analysis (SCAN) workflow and develop a frequency-potency algorithm to estimate B cell frequencies at various neutralizing activity or binding affinity cutoffs. In an HIV-1 fusion peptide (FP) immunization study, frequency-potency curves elucidate the quantity and quality of FP-specific immunoglobulin G (IgG)+ memory B cells for different animals, time points, and antibody lineages at single-cell resolution. The BCR neutralizing activities are mainly determined by their affinities to soluble envelope trimer. Frequency analysis definitively demonstrates dominant neutralizing antibody lineages. These findings establish SCAN and frequency-potency analyses as promising approaches for general B cell analysis and monoclonal antibody (mAb) discovery. They also provide specific rationales for HIV-1 FP-directed vaccine optimization.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11003769 | PMC |
| http://dx.doi.org/10.1016/j.celrep.2024.113948 | DOI Listing |
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