AI Article Synopsis
- Plant roots have different structures and functions, which might affect how bacteria grow on them.
- Researchers created two special setups to study different parts of plant roots and found that different types of bacteria live in different sections of the roots.
- They discovered that certain plant sugars help the bacteria thrive in specific areas, showing how plant nutrients and bacteria depend on each other for a healthy ecosystem.
Article Abstract
Plant roots are functionally heterogeneous in cellular architecture, transcriptome profile, metabolic state, and microbial immunity. We hypothesized that axial differentiation may also impact spatial colonization by root microbiota along the root axis. We developed two growth systems, ArtSoil and CD-Rhizotron, to grow and then dissect Arabidopsis thaliana roots into three segments. We demonstrate that distinct endospheric and rhizosphere bacterial communities colonize the segments, supporting the hypothesis of microbiota differentiation along the axis. Root metabolite profiling of each segment reveals differential metabolite enrichment and specificity. Bioinformatic analyses and GUS histochemistry indicate microbe-induced accumulation of SWEET2, 4, and 12 sugar uniporters. Profiling of root segments from sweet mutants shows altered spatial metabolic profiles and reorganization of endospheric root microbiota. This work reveals the interdependency between root metabolites and microbial colonization and the contribution of SWEETs to spatial diversity and stability of microbial ecosystem.
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| http://dx.doi.org/10.1016/j.chom.2024.02.014 | DOI Listing |
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