The type 9 secretion system (T9SS) is a recently discovered machinery that both transports cargo proteins across the Gram-negative bacterial outer membrane and attaches them to lipopolysaccharides on the extracellular surface. Outer membrane proteins (OMPs) are key components of the T9SS and are involved in both steps. In this chapter, we describe a method for the in silico modeling of T9SS OMPs and their complexes, and model validation. This is useful when the production of recombinant OMPs is difficult, and these protocols can also be applied to OMP complexes outside of the T9SS.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/978-1-0716-3734-0_20 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Comparative study of phenotypic and genotypic expression of virulence factors in colonizing and pathogenic carbapenem resistant Acinetobacter baumannii (CRAB).
BMC Microbiol
January 2025
Department of Pediatrics, Institute of Medical Sciences, Banaras Hindu University, Varanasi, India.
Carbapenem resistant Acinetobacter baumannii has evolved as the most troublesome microorganism with multiple virulence factors. Biofilm formation, porins, micronutrient capturing mechanism and quorum sensing, provide protection against desiccation, host-pathogen killing and enhance its persistence. The conservation of these factors between colonizing and pathogenic carbapenem resistant A.
View Article and Find Full Text PDFSynthesis, biological evaluation and validation of IMB-881 derivatives as anti-Gram-negative bacterial agents.
Bioorg Med Chem
January 2025
Beijing Key Laboratory of Technology and Application for Anti-Infective New Drugs Research and Development, Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China. Electronic address:
Infectious diseases caused by drug-resistant bacteria represent one of the most significant global public challenges of this century. There is an urgent need for the treatment of drug-resistant Gram-negative bacterial infections. A series of 3,4-dihydro-2H-[1,3]oxazino[5,6-h]quinoline derivatives were synthesized and evaluated for their antibacterial activity against Gram-negative bacteria including strains from ATCC and clinical isolates, initially revealing the structure-activity relationship.
View Article and Find Full Text PDFNano-viscosimetry analysis of membrane disrupting peptide magainin2 interactions with model membranes.
Biophys Chem
January 2025
La Trobe Institute for Molecular Science, La Trobe University, Melbourne, Australia. Electronic address:
The rapid spread of antibiotic-resistant strains of bacteria has created an urgent need for new alternative antibiotic agents. Membrane disrupting antimicrobial peptides (AMPs): short amino acid sequences with bactericidal and fungicidal activity that kill pathogens by permeabilizing their plasma membrane may offer a solution for this global health crisis. Magainin 2 is an AMP secreted by the African clawed frog (Xenopus laevis) that is described as a toroidal pore former membrane disrupting AMP.
View Article and Find Full Text PDFGet the Ball Rolling: Update and Perspective on the Role of Chloroplast Plastoglobule-associated Protein under Abiotic Stress.
J Exp Bot
January 2025
Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing, Michigan USA.
Plastid-localized plastoglobules (PGs) are monolayer lipid droplets typically associated with the outer envelope of thylakoid membranes in chloroplasts. The size and number of PGs can vary significantly in response to different environmental stimuli. Since the early 21st century, a variety of proteins attached to the surface of PGs have been identified and experimentally characterized using advanced biotechnological techniques, revealing their biological functions.
View Article and Find Full Text PDFMitochondrial Porin Is Required for Versatile Biocontrol Trait-Involved Biological Processes in a Filamentous Insect Pathogenic Fungus.
J Agric Food Chem
January 2025
Key Laboratory of Agricultural Biosafety and Green Production of Upper Yangtze River (Ministry of Education), College of Plant Protection, Southwest University, Chongqing 400715, China.
The mitochondrial voltage-dependent anion channel (VDAC) is the major channel in the mitochondrial outer membrane for metabolites and ions. VDACs also regulate a variety of biological processes, which vary in the number of VDAC isoforms across different eukaryotes. However, little is known about VDAC-mediated biocontrol traits in biocontrol fungi.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!