Plants (Basel)
Laboratory of Plant Nematology, South China Agricultural University, Guangzhou 510642, China.
Published: March 2024
is one of the most widespread and economically important sedentary endoparasites. In this study, a comparative transcriptome analysis of between pre-parasitic second-stage juveniles (Pre-J2) and parasitic juveniles (Par-J3/J4) was conducted. A total of 48,698 unigenes were obtained, of which 18,826 genes showed significant differences in expression ( < 0.05). In the differentially expressed genes (DEGs) from transcriptome data at Par-J3/J4 and Pre-J2, a large number of unigenes were annotated to the C-type lectin (CTL, ), the cathepsin L-like protease (), the venom allergen-like protein (), and the cellulase (endo-β-1,4-glucanase). Among seven types of lectins found in the DEGs, there were 10 CTLs. The regulatory roles of , and in plant immune responses involved in the parasitism of were investigated. The results revealed that could suppress programmed cell death (PCD) triggered by Gpa2/RBP-1 and inhibit the flg22-stimulated ROS burst. In situ hybridization and developmental expression analyses showed that was specifically expressed in the subventral gland of , and its expression was up-regulated at Pre-J2 of the nematode. In addition, silencing of substantially increased the plant resistance to . Moreover, yeast co-transformation and bimolecular fluorescence complementation assay showed that specifically interacted with the catalase, . It was demonstrated that could suppress the innate immunity of plants through the peroxide system, thereby promoting parasitism.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10935113 | PMC |
http://dx.doi.org/10.3390/plants13050730 | DOI Listing |
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