Characterization of the Effects of a Novel Probiotic on Colonization of a Piglet-Derived Intestinal Microbiota Using Improved Bioreactor.

The carriage of in pigs is a major concern for the agri-food industry and for global healthcare systems. Humans could develop salmonellosis when consuming contaminated pig products. On the other hand, some serotypes could cause disease in swine, leading to economic losses on farms. The purpose of the present study was to characterize the anti- activity of a novel -based probiotic using a bioreactor containing a piglet-derived intestinal microbiota. Two methods of probiotic administration were tested: a single daily and a continuous dose. enumeration was performed using selective agar at T24h, T48h, T72h, T96h and T120h. The DNA was extracted from bioreactor samples to perform microbiome profiling by targeted 16S rRNA gene sequencing on Illumina Miseq. The quantification of short-chain fatty acids (SCFAs) was also assessed at T120h. The probiotic decreased counts at T96 for the daily dose and at T120 for the continuous one. Both probiotic doses affected the alpha and beta diversity of the piglet-derived microbiota ( < 0.05). A decrease in acetate concentration and an increase in propionate proportion were observed in the continuous condition. In conclusion, the tested -based product showed a potential to modulate microbiota and reduce colonization in a piglet-derived intestinal microbiota and could therefore be used in vivo.