Antioxidant and Angiotensin-Converting Enzyme Inhibitory Activity of Faba Bean-Derived Peptides After Gastrointestinal Digestion: Insight into Their Mechanism of Action.

Faba bean flour, after gastrointestinal digestion, showed important antioxidant and angiotensin-converting enzyme (ACE) inhibitory activities. In the present study, 11 faba bean- derived peptides were synthesized to confirm their bioactivities and provide a deeper understanding of their mechanisms of action. The results revealed that 7 peptides were potent antioxidants, namely, NYDEGSEPR, TETWNPNHPEL, TETWNPNHPE, VIPTEPPH, VIPTEPPHA, VVIPTEPPHA, and VVIPTEPPH. Among them, TETWNPNHPEL had the highest activity in the ABTS (EC = 0.5 ± 0.2 mM) and DPPH (EC = 2.1 ± 0.1 mM) assays ( < 0.05), whereas TETWNPNHPE had the highest activity ( < 0.05) in the ORAC assay (2.84 ± 0.08 mM Trolox equivalent/mM). Synergistic and/or additive effects were found when selected peptides (TETWNPNHPEL, NYDEGSEPR, and VVIPTEPPHA) were combined. Four peptides were potent ACE inhibitors, where VVIPTEPPH (IC = 43 ± 1 μM) and VVIPTEPPHA (IC = 50 ± 5 μM) had the highest activity ( < 0.05), followed by VIPTEPPH (IC = 90 ± 10 μM) and then VIPTEPPHA (IC = 123 ± 5 μM) ( < 0.05). These peptides were noncompetitive inhibitors, as supported by kinetic studies and a molecular docking investigation. This study demonstrated that peptides derived from faba beans have multifunctional bioactivities, making them a promising food-functional and nutraceutical ingredient.