AI Article Synopsis
- Antimicrobial peptides (AMPs) like cecropin B show potential against antibiotic-resistant bacteria, but these pathogens have developed various defense mechanisms against AMPs.
- Researchers conducted single-cell analyses on bacterial response to cecropin B, observing significant variability in cell survival that suggests a phenomenon known as heteroresistance.
- Three genetic factors were identified as contributors to AMP resistance: the PhoPQ system, OmpT-induced cleavage of cecropin, and Rcs-mediated membrane stress response, indicating that bacteria use diverse strategies to resist antimicrobial peptides.
Article Abstract
Antimicrobial peptides (AMPs) are a promising tool with which to fight rising antibiotic resistance. However, pathogenic bacteria are equipped with several AMP defense mechanisms, whose contributions to AMP resistance are often poorly defined. Here, we evaluate the genetic determinants of resistance to an insect AMP, cecropin B, in the opportunistic pathogen . Single-cell analysis of response to cecropin revealed marked heterogeneity in cell survival, phenotypically reminiscent of heteroresistance (the ability of a subpopulation to grow in the presence of supra-MIC concentration of antimicrobial). The magnitude of this response was highly dependent on initial inoculum. We identified 3 genetic factors which collectively contribute to resistance in response to the AMP cecropin: The PhoPQ-two-component system, OmpT-mediated proteolytic cleavage of cecropin, and Rcs-mediated membrane stress response. Altogether, this evidence suggests that multiple, independent mechanisms contribute to AMP resistance in .
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10925329 | PMC |
| http://dx.doi.org/10.1101/2024.03.03.583169 | DOI Listing |
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