AI Article Synopsis
- Aflatoxin B is highly toxic and poses health risks, prompting the development of a sensitive detection system called CAFI using a click reaction approach.
- The detection process involves a copper-based metal-organic framework (Cu-MOF) to capture aflatoxin, which subsequently facilitates a chemical reaction that produces a fluorescence signal for measurement.
- This automated immunosensor achieves a detection limit of 0.48 pg/mL and is 670 times more sensitive than traditional ELISA methods, showcasing its promising potential for practical use.
Article Abstract
Due to the high toxicity of aflatoxin B and its risks to human health, we developed a click reaction-mediated automated fluorescent immunosensor (CAFI) for sensitive detection of aflatoxin B based on the Cu(I)-catalyzed click reaction. With its large specific surface area, a copper-based metal-organic framework (Cu-MOF) was synthesized to adsorb and enrich the copper ion (Cu(II)) and then load the complete antigen (BSA-AFB). After the immunoreaction, Cu(II) inside the Cu-MOF-Antigen conjugate would be reduced to Cu(I) in the presence of sodium ascorbate, which triggered the click reaction between the fluorescent donor-modified DNA and the receptor-modified complementary DNA to lead to a fluorescence signal readout. The whole reaction steps were finished by the self-developed automated immunoreaction device. This CAFI method showed a limit of detection (LOD) of 0.48 pg/mL as well as a 670-fold enhancement in sensitivity compared to conventional ELISA, revealing its great potential in practical applications and automated detection.
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| http://dx.doi.org/10.1021/acs.jafc.3c09730 | DOI Listing |
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