AI Article Synopsis
- - The study focuses on the ube3a gene in Japanese flounder, a member of the E3 ubiquitin ligase family, and its potential role in fighting off Chinook salmon bafnivirus (CSBV).
- - Researchers cloned the ube3a gene, noting its high similarity (94%) to the Atlantic halibut and found that this gene is most expressed in the fin, gills, and skin of the fish.
- - In experiments, overexpressing ube3a led to a reduced viral load in infected gill cells, while blocking its function increased the viral load, indicating that ube3a likely has a significant antiviral effect through the ubiquitination pathway.
Article Abstract
Ube3a is a member of the E3 ubiquitin ligase HECTc family, and its role has been established in neurodevelopmental disorders. However, studies on its role in Japanese flounder are scarce. Thus, in this study, the ube3a of Japanese flounder was cloned, and its role in conferring resistance against Chinook salmon bafnivirus (CSBV) was analyzed. Japanese flounder ube3a encoded a protein containing 834 amino acids. Interestingly, its homology with the Atlantic halibut was determined to be 94%. In addition, there were differential expressions of ube3a in different tissues of Japanese flounder, with the highest expression level observed in the fin, followed by the gills and skin (P ≤ 0.05). Subcellular localization analysis revealed that Ube3a is a cytoplasmic protein. We established an in vitro CSBV infection model using Japanese flounder gill cell line (FG). After ube3a overexpression, the viral load was significantly lower than that of the control group (P ≤ 0.05). Contrastingly, after incubation of FG cells with an E3 ubiquitin ligase inhibitor, the viral load was significantly higher than in the control group (P ≤ 0.01). Then, the expression levels of nf-κb, traf3, and tnf-α after incubation with an E3 ubiquitin ligase inhibitor were examined. The results demonstrated that ube3a may exerted a significant antiviral effect in Japanese flounder via the ubiquitination pathway.
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| http://dx.doi.org/10.1016/j.fsi.2024.109495 | DOI Listing |
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