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Single-Animal, Single-Tube RNA Extraction for Comparison of Relative Transcript Levels via qRT-PCR in the Tardigrade Hypsibius exemplaris.
J Vis Exp
January 2025
Department of Molecular Cellular and Developmental Biology, University of California, Santa Barbara; Neuroscience Research Institute, University of California, Santa Barbara.
The tardigrade Hypsibius exemplaris is an emerging model organism renowned for its ability to survive environmental extremes. To explore the molecular mechanisms and genetic basis of such extremotolerance, many studies rely on RNA-sequencing (RNA-seq), which can be performed on populations ranging from large cohorts to individual animals. Reverse transcription polymerase chain reaction (RT-PCR) and RNA interference (RNAi) are subsequently used to confirm RNA-seq findings and assess the genetic requirements for candidate genes, respectively.
View Article and Find Full Text PDFConstruction of bimetallic oxy-hydroxides based on Ni(OH) nanosheets for sensitive non-enzymatic glucose detection electrochemical oxidation and incorporation.
Nanoscale
January 2025
School of Materials Science and Engineering, Jiangsu University of Science and Technology, Zhenjiang 212003, China.
Due to their ease of synthesis and large specific surface area, Ni(OH) nanosheets have emerged as promising electrochemical sensing materials, attracting significant attention in recent years. Herein, a series of oxy-hydroxides based on Ni(OH) nanosheets, including NiO/Ni(OH)@NF and (MNi)O/Ni(OH)@NF (M = Co, Fe, or Cr), are successfully synthesized the electrochemical oxidation and incorporation strategies. Electrochemical tests demonstrate that these Ni(OH)-based oxy-hydroxides exhibit excellent electrochemical oxidation activity for glucose in alkaline electrolyte.
View Article and Find Full Text PDFAnti-freezing conductive hydrogels with exceptional mechanical properties and stable sensing performance at -30 °C.
Mater Horiz
January 2025
Tianjin Key Laboratory of Composite and Functional Materials, School of Materials Science and Engineering, Tianjin University, Tianjin, 300350, P. R. China.
Conductive hydrogels with stable sensing performance are highly required in soft electronic devices. However, these hydrogels tend to solidify and experience structural damage at sub-zero temperatures, leading to material breakdown and device malfunction. The main challenge lies in effectively designing the micro/nano-structure to enhance mechanical properties and stable strain sensing while preventing freezing in hydrogels.
View Article and Find Full Text PDFRhodamine based turn-on dual mode chemosensor for the selective recognition of nickel ions: practical and theoretical applications.
RSC Adv
January 2025
Bioorganic Laboratory, Department of Chemistry, University of Delhi Delhi-110007 India
This work presents the development of a rhodamine-based colorimetric and turn-on fluorescent chemosensor (P1) designed for selective recognition of Ni ions. Chemosensor P1 exhibited remarkable sensitivity and selectivity for Ni ions, exhibiting clear colorimetric and fluorescence responses. The binding interactions were meticulously examined using UV-Vis.
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