AI Article Synopsis
- Bemisia tabaci is a highly damaging pest that affects various crops, and its management is complicated by pesticide resistance.
- The study focused on the Ecdysis triggering hormone receptor (ETHr) gene, finding that it is most expressed in third-instar nymphs and plays a critical role in the growth and reproduction of whiteflies.
- Silencing the ETHr gene using RNA interference (RNAi) resulted in significantly higher mortality rates among whiteflies and reduced their reproductive success, suggesting that targeting this gene could lead to new insecticides for controlling whitefly populations.
Article Abstract
Bemisia tabaci (Gennadius) is one of the most dangerous polyphagous pests in the world causing damage to various crops by sucking sap during the nymphal and adult stages. Chemical management of whiteflies is challenging because of the emergence of pesticide resistance. RNA interference has been well established in whitefly to study the functions of various genes. G-protein coupled receptors (GPCRs) are important targets for development of new generation insecticides. In this study, Ecdysis triggering hormone receptor (ETHr) gene expression was recorded in different stages of whitefly and its function has been studied through RNAi. The expression of ETHr is highest in third-instar nymphs followed by other nymphal instars, pupae and newly emerged adults. Silencing of ETHr resulted in significantly higher adult mortality (68.88%), reduced fecundity (4.46 eggs /female), reduced longevity of male and female (1.05 and 1.40 days, respectively) when adults were fed with dsETHr @ 1.0 μg/μl. Silencing of ETHr in nymphs lead to significantly higher mortality (81.35%) as compared to control. This study confirms that ETHr gene is essential for growth and development of whitefly nymphs and adults. Hence, it can be future target for developing dsRNA based insecticides for management of whitefly.
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| http://dx.doi.org/10.1016/j.pestbp.2023.105766 | DOI Listing |
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