AI Article Synopsis
- Study aimed to find Mycobacterium tuberculosis (Mtb) DNA in blood cells of TB patients and those with TB infection in Italy.
- Research involved 57 TB patients, 41 with TB infection, and 39 controls, using advanced DNA detection methods on blood samples.
- Results showed low levels of Mtb DNA in various groups, highlighting a potential link between CD34 cells and Mtb, which could aid in understanding TB and developing new diagnostic tools.
Article Abstract
Objectives: To investigate whether Mycobacterium tuberculosis (Mtb) DNA is detected in peripheral blood mononuclear cells (PBMC) of subjects with tuberculosis (TB) or TB infection (TBI) living in a low-burden country.
Methods: We prospectively enrolled 57 patients with TB, 41 subjects with TBI, and 39 controls in Rome, Italy. PBMC were isolated, cluster of differentiation (CD)34 and CD34 cells were immunomagnetic separated, DNA was extracted, and digital polymerase chain reaction for IS6110 and rpoB sequences was used to detect Mtb DNA in PBMC subsets and unfractionated PBMC.
Results: We detected Mtb DNA at a low copy number in CD34 cells in 4o f 30 (13%) patients with TB, 2 of 24 (8%) subjects with TBI, and 1 of 24 (4%) controls. Mtb DNA was detected in unfractionated PBMC in 3 of 51 (6%) patients with TB, 2 of 38 (5%) subjects with TBI, and 2 of 36 (6%) controls. In CD34 cells, only 1 of 31 (3%) subjects with TBI tested positive for Mtb DNA.
Conclusions: Mtb DNA was detected at low frequencies and levels in the PBMC of subjects with TBI and donors with TB living in a low-burden country. In particular, Mtb DNA was detected more frequently in CD34 cells, supporting the hypothesis that these cells may represent a Mtb niche. This finding informs biological understanding of Mtb pathogenesis and may support the development of a microbial blood biomarker for Mtb infection.
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| http://dx.doi.org/10.1016/j.ijid.2024.106999 | DOI Listing |
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