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Digital CRISPR-Powered Biosensor Concept without Target Amplification Using Single-Impact Electrochemistry.
ACS Sens
November 2024
Neuroelectronics, Munich Institute of Biomedical Engineering, Department of Electrical Engineering, School of Computation, Information and Technology, Technical University of Munich, 85748 Garching, Germany.
The rapid and reliable detection and quantification of nucleic acids is crucial for various applications, including infectious disease and cancer diagnostics. While conventional methods, such as the quantitative polymerase chain reaction are widely used, they are limited to the laboratory environment due to their complexity and the requirement for sophisticated equipment. In this study, we present a novel amplification-free digital sensing strategy by combining the collateral cleavage activity of the Cas12a enzyme with single-impact electrochemistry.
View Article and Find Full Text PDFCRISPR-Powered Strategies for Amplification-Free Diagnostics of Infectious Diseases.
Anal Chem
May 2024
State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, Key Laboratory of Magnetic Resonance in Biological Systems, National Center for Magnetic Resonance in Wuhan, Wuhan Institute of Physics and Mathematics, Innovation Academy for Precision Measurement Science and Technology, Chinese Academy of Sciences - Wuhan National Laboratory for Optoelectronics, Wuhan 430071, China.
Exploring nano-enabled CRISPR-Cas-powered strategies for efficient diagnostics and treatment of infectious diseases.
J Nanostructure Chem
February 2022
NanoBioTech Laboratory, Health System Engineering, Department of Natural Sciences, Florida Polytechnic University, Lakeland, FL-33805 USA.
Unlabelled: Biomedical researchers have subsequently been inspired the development of new approaches for precisely changing an organism's genomic DNA in order to investigate customized diagnostics and therapeutics utilizing genetic engineering techniques. Clustered Regulatory Interspaced Short Palindromic Repeats (CRISPR) is one such technique that has emerged as a safe, targeted, and effective pharmaceutical treatment against a wide range of disease-causing organisms, including bacteria, fungi, parasites, and viruses, as well as genetic abnormalities. The recent discovery of very flexible engineered nucleic acid binding proteins has changed the scientific area of genome editing in a revolutionary way.
View Article and Find Full Text PDFAdvances in amplification-free detection of nucleic acid: CRISPR/Cas system as a powerful tool.
Anal Biochem
April 2022
College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou, 310058, China; Key Laboratory of on Site Processing Equipment for Agricultural Products, Ministry of Agriculture, Hangzhou, 310058, China. Electronic address:
Article Synopsis
- Amplification technologies like PCR are commonly used for detecting nucleic acids but have limitations, including the need for complex equipment and risks of false positives due to contamination.
- The CRISPR/Cas system is emerging as a powerful alternative for nucleic acid detection, utilizing its ability to degrade RNA or DNA quickly, enabling detection without prior amplification.
- Recent advances in CRISPR-based methods demonstrate comparable sensitivity to traditional amplification methods while addressing challenges in sample preparation, off-target effects, and quantitative detection, suggesting potential for rapid and cost-effective on-site testing.
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