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Evaluation of commercial immunodiffusion reagents for detecting serum anti-Paracoccidioides antibodies.
Rev Soc Bras Med Trop
May 2024
Universidade Federal do Paraná, Hospital de Clínicas, Departamento de Saúde Coletiva, Curitiba, PR, Brasil.
Article Synopsis
- - The study focuses on improving diagnosis of paracoccidioidomycosis through Paracoccidioides antibody detection using double immunodiffusion (DID), noting that testing effectiveness can vary based on specific conditions.
- - The researchers tested DID with 40 serum samples, finding it had a sensitivity of 90% and a perfect specificity of 100% for identifying the disease.
- - The results indicate that commercially prepared reagents for DID could be a reliable method for detecting anti-Paracoccidioides antibodies in patients.
Mycological diagnosis of paracoccidioidomycosis in a hospital from a nonendemic area: classical and molecular methods.
Biomedica
August 2023
Departamento de Micología, INEI-ANLIS "Dr. Carlos G. Malbrán", Ciudad Autónoma de Buenos Aires, Argentina.
Introduction: Paracoccidioidomycosis is a systemic mycosis endemic in Latin America. Climate change and host migration emphasize the need to optimize this infection diagnosis.
Objective: To evaluate the implementation of Paracoccidioides spp.
Estrogen Receptor-α Correlates with Higher Fungal Cell Number in Oral Paracoccidioidomycosis in Women.
Mycopathologia
October 2018
Department of Clinic and Surgery, School of Dentistry, Federal University of Alfenas, Rua Gabriel Monteiro da Silva 700, Alfenas, MG, 37130-001, Brazil.
Background: Paracoccidioidomycosis is a neglected tropical fungal infection with great predilection for adult men, indicating the participation of female hormone estrogen in preventing paracoccidioidomycosis development in women. Estrogen has an immunologic effect leading to polarization toward the Th2 immune response, which favors the disease evolution.
Objectives: To evaluate estrogen and progesterone receptors in oral paracoccidioidomycosis lesions and to verify any association with tissue fungi counting in women and men.
Patients with chronic-form paracoccidioidomycosis present high serum levels of IgE anti-paracoccidioides brasiliensis Gp70.
Mycopathologia
April 2013
Department of Pathologic Sciences, State University of Londrina (UEL), Londrina, PR, Brazil.
Paracoccidioidomycosis (PCM) is a disease caused by the Paracoccidioides genus, which includes P. brasiliensis and the new phylogenetic species P. lutzii.
View Article and Find Full Text PDFSerological diagnosis of paracoccidioidomycosis in HIV-coinfected patients.
Mem Inst Oswaldo Cruz
November 2010
Departamento de Clínica Médica, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brasil.
Paracoccidioidomycosis should be differentiated from other opportunistic diseases in human immunodeficiency virus (HIV)/acquired immunodeficiency syndrome (AIDS) patients who live in Latin America. Laboratory investigation can begin with serological tests, which are rapid and efficient. In the present study, double immunodiffusion (DID), counterimmunoelectrophoresis (CIEP) and an enzyme linked immunosorbent assay (ELISA) tests were assessed for the detection of anti-Paracoccidioides brasiliensis antibodies in 40 patients coinfected with HIV.
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