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Investigation of Sensitivity of Rapid Diagnosis Tests in Patients with Suspected Malaria. | LitMetric

AI Article Synopsis

  • In Türkiye, malaria was eradicated in 2010, but there are still imported cases, leading researchers to compare two rapid diagnostic tests for malaria detection: SD-Pf/Pan and SD-Pf/Pv, against microscopy and RT-PCR.
  • The study involved testing blood samples from 66 suspected malaria cases, with microscopy and RT-PCR confirming 30 positive cases, while both rapid tests showed high sensitivity and specificity results, with SD-Pf/Pan performing slightly lower on some measures.
  • The findings suggest that both rapid tests can effectively diagnose malaria, but results should be validated with microscopy and RT-PCR for accurate diagnosis.

Article Abstract

Objective: Malaria has been eradicated in Türkiye as of 2010, but there are imported cases. In this study, we aimed to compare the diagnostic value of two rapid tests; SD Bioline Malaria Ag Pf/Pan (SD-Pf/Pan) and SD Bioline Malaria Ag Pf/Pv (SD-Pf/Pv) with microscopy and real time-polymerase chain reaction (RT-PCR).

Methods: Blood samples were taken from all participants. Thick drop smears were prepared. Thick drop smears were examined for malaria positive/negative distinction under the light microscopy. Then, two rapid diagnostic tests (SD-Pf/Pan and SD-Pf/Pv) were performed. After DNA extraction from blood samples, RT-PCR was typed. The data were evaluated with SPSS 21 program of statistics.

Results: A total of 30 cases out of 66 suspected malaria cases were detected as positive with microscopy and RT-PCR. Twenty-seven patients were found positive with both SD-Pf/Pan and SD-Pf/Pv tests. Based on the microscopic results as a reference method, SD-Pf/Pan and SD-Pf/Pv rapid diagnostic tests had a 90% sensitivity, 100% specificity, 100% positive predictive value (PPV), and 92.86% negative predictive value (NPV). Based on the RT-PCR results as a reference method, for detection of , both tests had a 95.65% sensitivity, 100% specificity, 100% PPV, and 88.89% NPV. Moreover, while SD-Pf/Pv had a sensitivity, specificity, PPV, and NPV of 100% in detection of ; SD-Pf/Pan has a 77.78% sensitivity of, 61.90% specificity of, 46.67% PPV, and 86.67% NPV SD-Pf/Pan for detection of PAN.

Conclusion: As a result, high sensitivity and specificity were detected in both kits in the diagnosis of malaria infections caused by and . Rapid diagnostic tests can be used safely in diagnosis however the diagnosis should be supported by microscopy and RT-PCR methods when they are applicable.

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Source
http://dx.doi.org/10.4274/tpd.galenos.2024.38358DOI Listing

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