AI Article Synopsis
- X-ray computed tomography (XCT) and X-ray fluorescence (XRF) are non-invasive techniques for studying cells, but combining them for the same cell has been challenging due to preparation and damage issues.
- A new workflow has been developed for correlating XCT and XRF imaging by preparing radiation-resistant single-cell samples, allowing effective targeting and labeling for precise measurements.
- This integrated approach successfully visualizes cellular structures and chemical distributions, enhancing the understanding of cellular processes and the roles of elements and proteins in biology.
Article Abstract
X-ray computed tomography (XCT) and X-ray fluorescence (XRF) imaging are two non-invasive imaging techniques to study cellular structures and chemical element distributions, respectively. However, correlative X-ray computed tomography and fluorescence imaging for the same cell have yet to be routinely realized due to challenges in sample preparation and X-ray radiation damage. Here we report an integrated experimental and computational workflow for achieving correlative multi-modality X-ray imaging of a single cell. The method consists of the preparation of radiation-resistant single-cell samples using live-cell imaging-assisted chemical fixation and freeze-drying procedures, targeting and labeling cells for correlative XCT and XRF measurement, and computational reconstruction of the correlative and multi-modality images. With XCT, cellular structures including the overall structure and intracellular organelles are visualized, while XRF imaging reveals the distribution of multiple chemical elements within the same cell. Our correlative method demonstrates the feasibility and broad applicability of using X-rays to understand cellular structures and the roles of chemical elements and related proteins in signaling and other biological processes.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10917812 | PMC |
| http://dx.doi.org/10.1038/s42003-024-05950-y | DOI Listing |
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