Context: Resistance to antimalarial drugs is one of the major challenges for malaria elimination. In India, artemisinin combination therapy (artesunate-sulfadoxin pyrimethamine) was introduced in place of chloroquine (CQ) for the treatment of uncomplicated falciparum malaria in 2010. Periodical monitoring of polymorphisms in antimalarial drug resistance marker genes will be useful for assessing drug pressure, mapping and monitoring of drug resistance status; and will be helpful for searching alternative treatments.
Objectives: This study was conducted to study the polymorphisms in antimalarial drug resistance marker genes among clinical isolates collected from Kolkata after 10 years of artemisinin-based combination therapie (ACT) implementation.
Materials And Methods: Blood samples were collected from mono-infected patients and polymorphisms in CQ resistance transporter , multidrug resistance , dihydrofolate reductase , dihydropteroate synthetase , and propeller genes were analysed by polymerase chain reaction and DNA sequencing.
Results: In gene, C72S, and K76T mutation was recorded in 100% isolates and no mutations was detected in any of the targeted codons of gene. A double mutant haplotype SVMNT and wildtype haplotype NYD in were prevalent in 100% of study isolates. Triple mutant haplotype ANRNI-SGKAA was recorded. No polymorphism in gene was documented in any of the isolates.
Conclusions: Observed wild codon N86 along with Y184 and D1246 of gene might be an indication of the reappearance of CQ sensitivity. The absence of quadruple and quintuple haplotypes in gene along with the wild haplotype of pfK13 is evidence of ACT effectivity. Hence, similar studies with large sample size are highly suggested for monitoring the drug resistance status of .
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10911185 | PMC |
| http://dx.doi.org/10.4103/tp.tp_43_23 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Ergosterol alleviates hepatic steatosis and insulin resistance via promoting fatty acid β-oxidation by activating mitochondrial ACSL1.
Cell Rep
January 2025
State Key Laboratory of Natural Medicines, School of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing, Jiangsu, China. Electronic address:
Sterols target sterol-sensing domain (SSD) proteins to lower cholesterol and circulating and hepatic triglyceride levels, but the mechanism remains unclear. In this study, we identify acyl-coenzyme A (CoA) synthetase long-chain family member 1 (ACSL1) as a direct target of ergosterol (ES). The C-terminal domain of ACSL1 undergoes conformational changes from closed to open, and ES may target the drug-binding pocket in the acetyl-CoA synthetase-like domain 1 (ASLD1) of ACSL1 to stabilize the closed conformation and maintain its activity.
View Article and Find Full Text PDFMorbidity and mortality associated with ESBL Klebsiella pneumoniae infection in different administration routes in albino rats.
Cell Mol Biol (Noisy-le-grand)
January 2025
Department of Medical Microbiology, Faculty of Science and Health, Koya University, Koya KOY45, Kurdistan Region-F.R., Iraq.
Klebsiella pneumoniae is a non-motile, encapsulated, environmental gram-negative bacterium. Once the bacteria have infiltrated the body, they can display substantial degrees of resistance to drugs and virulence. Extended Spectrum Beta-Lactamases (ESBLs) are most typically seen in K.
View Article and Find Full Text PDFAssessment of bacteriological and immunological markers in urinary tract infection and the effect of antibiotics on the isolated bacteria.
Cell Mol Biol (Noisy-le-grand)
January 2025
Laboratory of Plant Improvement and Valorization of Agro-resources, National School of Engineers of Sfax, University of Sfax, Sfax LR.16ES20, Tunisia.
Urinary tract infections (UTIs) are recognized as the second most common medical condition, following respiratory infections. Despite the availability of numerous efficacious antibiotics for the management of UTIs, the rising incidence of bacterial resistance presents significant challenges in the treatment of these infections. Bacteria are endowed with the ability to reproduce and develop resistance mechanisms against antibiotics.
View Article and Find Full Text PDFMechanisms and implications of antibiotic resistance in gram-positive bacterial strains.
Cell Mol Biol (Noisy-le-grand)
January 2025
Department of Pharmaceutical Sciences, College of Pharmacy, Princess Nourah Bint Abdulrahman University, Riyadh, Saudi Arabia.
Antibiotics play a fundamental role in protecting millions of lives from infectious diseases. However, an important drawback of antibiotic treatment is that each advancement was followed by the development of resistance. This is due to the fact that the majority of pathogenic bacteria are capable of becoming resistant to a number of antimicrobial agents.
View Article and Find Full Text PDFAntimicrobial activity of Enterococcus-derived bacteriocins against multidrug-resistant Pseudomonas aeruginosa.
Cell Mol Biol (Noisy-le-grand)
January 2025
Department Medical Laboratory Technology, College of Medical Technology, University of Al-Farahidi, Baghdad, Iraq.
Article Synopsis
- Pseudomonas aeruginosa is problematic in healthcare due to its high antibiotic resistance, highlighting the need for new antimicrobial solutions.
- A study focused on isolating a new bacteriocin from Enterococcus faecium found in stool samples, which showed promise against multidrug-resistant P. aeruginosa.
- The purified bacteriocin, enterocin GH, demonstrated significant antibacterial and antibiofilm activity against P. aeruginosa, outperforming controls in laboratory tests.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!