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Human keratinocytes grown at a gas-permeable interface in vitro stratify correctly to generate engineered human epidermis.
Cytotherapy
December 2024
School of Biological Sciences, University of Auckland, Auckland, New Zealand; Maurice Wilkins Centre, The University of Auckland, Auckland, New Zealand. Electronic address:
Background: One of the key functions of human skin is to provide a barrier, protecting the body from the surrounding environment and maintaining homeostasis of the internal environment. A mature, stratified epidermis is critical to achieve skin barrier function and is particularly important when producing skin grafts in vitro for wound treatment. For decades epidermal stratification has been achieved in vitro by culturing keratinocytes at an air-liquid interface, triggering proliferating basal keratinocytes to differentiate and form all epidermal layers.
View Article and Find Full Text PDFSpatiotemporal Monitoring of Cropland Soil Organic Carbon Changes From Space.
Glob Chang Biol
December 2024
Thünen Earth Observation (ThEO), Thünen Institute of Farm Economics, Braunschweig, Germany.
Soil monitoring requires accurate and spatially explicit information on soil organic carbon (SOC) trends and changes over time. Spatiotemporal SOC models based on Earth Observation (EO) satellite data can support large-scale SOC monitoring but often lack sufficient temporal validation based on long-term soil data. In this study, we used repeated SOC samples from 1986 to 2022 and a time series of multispectral bare soil observations (Landsat and Sentinel-2) to model high-resolution cropland SOC trends for almost four decades.
View Article and Find Full Text PDFA Technology System to Help People With Intellectual Disability and Blindness Find Room Destinations During Indoor Traveling: Case Series Study.
JMIR Rehabil Assist Technol
November 2024
Department of Psychology, Sigmund Freud University, Milan, Italy.
Background: People with severe or profound intellectual disability and visual impairment tend to have serious problems in orientation and mobility and need assistance for their indoor traveling. The use of technology solutions may be critically important to help them curb those problems and achieve a level of independence.
Objective: This study aimed to assess a new technology system to help people with severe to profound intellectual disability and blindness find room destinations during indoor traveling.
Rho1 and Rgf1 establish a new actin-dependent signal to determine growth poles in yeast independently of microtubules and the Tea1-Tea4 complex.
PLoS Biol
November 2024
Instituto de Biología Funcional y Genómica (IBFG), CSIC/Universidad de Salamanca and Departamento de Microbiología y Genética, Universidad de Salamanca. C/ Zacarías González, Salamanca, Spain.
Cellular asymmetry begins with the selection of a discrete point on the cell surface that triggers Rho-GTPases activation and localized assembly of the cytoskeleton to establish new growth zones. The cylindrical shape of fission yeast is organized by microtubules (MT) that deliver the landmark Tea1-Tea4 complex at the cell tips to define the growth poles. However, only a few tea1Δ cells mistaken the direction of growth, indicating that they manage to detect their growth sites.
View Article and Find Full Text PDFStand-off hazardous materials identification based on near-infrared hyperspectral imaging combined with convolutional neural network.
Spectrochim Acta A Mol Biomol Spectrosc
February 2025
Institute of Biomedical Photonics and Sensing, Xi'an Jiaotong University, Xi'an, Shanxi 710049, China. Electronic address:
Near-infrared (NIR) hyperspectral imaging enables rapid, non-contact imaging of hazardous materials in a non-destructive manner, allowing for analysis based on spectral reflection information. However, using traditional methods, it is challenging to identify hazardous materials with less distinct spectral reflection features. This study utilizes a self-built NIR hyperspectral imaging system and proposes a new approach.
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