Highly selective nanozyme-based glucose sensing platform via construction of artificial recognition sites on gold nanospheres.

Biosens Bioelectron

Institute of Biomedical Engineering, College of Life Science, Qingdao University, Qingdao, 266071, China. Electronic address:

Published: June 2024

AI Article Synopsis

  • * This study focuses on improving the selectivity of Au-based nanozymes (PDA@Au NPs) by using molecularly imprinted polymers (MIPs) that target glucose, achieving a selectivity improvement of over 230%.
  • * The resulting glucose sensor is capable of detecting glucose in a concentration range of 10 μM-1 mM, with a low detection limit of 0.227 μM, while being unaffected by other monosaccharides and demonstrating good recovery and long-term stability.

Article Abstract

Nanozymes have been regarded as the ideal alternatives to natural enzymes in bioassays due to their good stability and low cost. However, their applications in sensing usually suffer from poor selectivity. For example, Au-based nanozymes, as a kind of classical glucose oxidase mimic enzyme, could catalyze diverse monosaccharides. Therefore, it is of great necessity and urgency to endow the Au-based nanozymes with enhanced selectivity for the construction of specific glucose sensing platform. In our study, easily recyclable polydopamine (PDA)-supported Au-based nanozymes (PDA@Au NPs) were successfully prepared and could catalyze diverse monosaccharides including glucose, xylose, mannose, and sucrose. To enhance the selectivity of PDA@Au NPs, molecularly imprinted polymers (MIPs) were constructed on the surface of PDA@Au NPs using glucose and boronic acid derivatives as template and functional monomer. Impressively, the catalytic activity of the obtained molecularly imprinted nanozyme (PDA@Au NPs-MIPs) only shows a slight decrease (6.3%) while their selectivity is obviously enhanced (≥230%). Accordingly, the as-prepared sensor achieved the sensitive and selective detection of glucose in the concentration range of 10 μM-1 mM and a low detection limit (LOD) of 0.227 μM (S/N = 3), avoiding the influence of other monosaccharides exited in the sensing solutions to a great extent. As expected, the as-prepared sensors also showed good recovery, and long-term stability.

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http://dx.doi.org/10.1016/j.bios.2024.116169DOI Listing

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Highly selective nanozyme-based glucose sensing platform via construction of artificial recognition sites on gold nanospheres.

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  • * The resulting glucose sensor is capable of detecting glucose in a concentration range of 10 μM-1 mM, with a low detection limit of 0.227 μM, while being unaffected by other monosaccharides and demonstrating good recovery and long-term stability.
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