Xanthine oxidase inhibition study of isolated secondary metabolites from (Bignoniaceae): and approach.

Xanthine oxidase (XO) has been widely recognized as a pivotal enzyme in developing hyperuricemia, primarily contributing to the excessive production of uric acid during purine metabolism in the liver. One of the standard treatment approaches involves reducing uric acid levels by inhibiting XO activity. In this study, the leaf extract of , traditionally used in folk medicine, was found to inhibit XO activity in the ethyl acetate and butanol fractions at a concentration of 100 µg/mL, their values were 78.57 ± 3.85 % (IC = 55.93 ± 5.73 µg/ml) and 69.43 ± 8.68 % (IC = 70.17 ± 7.98 µg/ml), respectively. The potential XO inhibitory components were isolated by bioactivity assays and the HR-ESI-MS and NMR spectra system. The main constituents of leaf extracts of , six compounds, namely 4-methoxycinnamic acid (), -3,4-dimethoxycinnamic acid (), -coumaric acid (), martynoside (), 6-O-(-methoxy--cinnamoyl)-ajugol (), and scolymoside (), were identified as potent XO inhibitors with IC values ranging from 19.34 ± 1.63 μM to 64.50 ± 0.94 μM. The enzyme kinetics indicated that compounds -, , and displayed competitive inhibition like allopurinol, while compound displayed a mixed-type inhibition. Computational studies corroborated these experimental results, highlighting the interactions between potential metabolites and XO enzyme. The hydrogen bonds played crucial roles in the binding interaction, especially, scolymoside () forms a hydrogen bond with Mos3004, exhibited the lowest binding energy (-18.3286 kcal/mol) corresponding to the lowest IC (19.34 ± 1.63 μM). Furthermore, nine compounds were isolated for the first time from this plant. , and its constituents possess the potential to modulate the xanthine oxidase enzyme involved in metabolism.