Tips and tools to obtain and assess mosquito viromes.

Arch Microbiol

Laboratory of Immunobiology and Immunogenetics, Department of Genetics, Postgraduate Program in Genetics and Molecular Biology (PPGBM), Universidade Federal do Rio Grande do Sul (UFRGS), UFRGS. Av. Bento Gonçalves, 9500, Porto Alegre, Rio Grande do Sul, Brazil.

Published: March 2024

AI Article Synopsis

  • Mosquitoes are important to study because they can spread dangerous diseases like dengue and yellow fever that affect people's health.
  • Researchers are looking at the viruses found in mosquitoes to help prevent and detect these diseases earlier.
  • This article explains the complicated steps involved in studying mosquito viruses, including capturing them, preparing samples, and analyzing the data.

Article Abstract

Due to their vectorial capacity, mosquitoes (Diptera: Culicidae) receive special attention from health authorities and entomologists. These cosmopolitan insects are responsible for the transmission of many viral diseases, such as dengue and yellow fever, causing huge impacts on human health and justifying the intensification of research focused on mosquito-borne diseases. In this context, the study of the virome of mosquitoes can contribute to anticipate the emergence and/or the reemergence of infectious diseases. The assessment of mosquito viromes also contributes to the surveillance of a wide variety of viruses found in these insects, allowing the early detection of pathogens with public health importance. However, the study of mosquito viromes can be challenging due to the number and complexities of steps involved in this type of research. Therefore, this article aims to describe, in a straightforward and simplified way, the steps necessary for obtention and assessment of mosquito viromes. In brief, this article explores: the capture and preservation of specimens; sampling strategies; treatment of samples before DNA/RNA extraction; extraction methodologies; enrichment and purification processes; sequencing choices; and bioinformatics analysis.

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Source
http://dx.doi.org/10.1007/s00203-023-03813-4DOI Listing

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